1999年12月
Dendritic cells stimulate the expansion of PML-RAR alpha specific cytotoxic T-lymphocytes: Its applicability for antileukemia immunotherapy
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
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- 巻
- 18
- 号
- 4
- 開始ページ
- 485
- 終了ページ
- 492
- 記述言語
- 英語
- 掲載種別
- 出版者・発行元
- APSIT ASSOC PROM STUD IMMUNOL TUMOR
Dendritic cells (DC), the most potent "professional" antigen-presenting cells, hold promise for improving the immunotherapy of cancer. In this study, we investigated the ability of normal donor DC pulsed ex vivo with 12 mer PML-RAR alpha (A) peptide (SGAGEAAIETQS) to generate peptide specific autologous cytotoxic T- lymphocytes. The peptide pulsed DC-primed peripheral blood lymphocytes (PBL) displayed significantly higher cytotoxic activity compared with that of peptide non-pulsed DC-primed PBL against peptide-pulsed autologous macrophages (P<0.001). Both CD8+ and CD4+ T lymphocytes were involved in the effector cell populations. The PML-RAR alpha peptide-pulsed DC-primed T-cells were significantly superior in their production of GM-CSF and TNF-alpha compared with peptide non-pulsed DC-primed T-cells, These intriguing preclinical studies, suggest that PML-RAR alpha pulsed-DC could be a promising immunotherapeutic modality for patients with acute promyelocytic leukemia.
- リンク情報
- ID情報
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- ISSN : 0392-9078
- Web of Science ID : WOS:000085400700008