2005年2月
Analysis of marginal zone B cell development in the mouse with limited B cell diversity: Role of the antigen receptor signals in the recruitment of B cells to the marginal zone
JOURNAL OF IMMUNOLOGY
- ,
- ,
- 巻
- 174
- 号
- 3
- 開始ページ
- 1438
- 終了ページ
- 1445
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- 出版者・発行元
- AMER ASSOC IMMUNOLOGISTS
The quasimonoclonal (QM) mouse provides an intelligible model to analyze the B cell selection as the competition between two major 4-hydroxy-3-nitrophenylacetyl-specific B cell populations whose BCR are comprised of the knockin V(H)17.2.25 (VHT)encoded H chain and the lambda1 or lambda2 L chain. In this study, we show the QM system is useful to examine how BCR signals guide a subset of B cells to the marginal zone (MZ). Compared with the control C57BL/6 mice, the QM mice had similar to2.7-fold increased number of B cells exhibiting the MZ B cell phenotype and a larger MZ area in the spleen. Interestingly, VHT/lambda2 B cells significantly predominated over VHT/lambda1 B cells in MZ-(VHT/lambda1:VHT/lambda2 approximate to 3:7) and transitional 2-B cell subsets, while these two populations were comparable in immature, transitional 1, and mature counterparts. Thus, the biased use of lambda2 in the MZ B cells may be the result of selection in the periphery. The enlargement of MZ B cell compartment and the preferred recruitment of the VHT/(lambda)2 B cells were further augmented by doubling the VHT gene, but dampened by the dysfunction of Bruton's tyrosine kinase, suggesting a positive role of BCR signaling in this selection. Comparison of Ag specificity between VHT/lambda1 and VHT/lambda2 IgM mAbs revealed a polyreactive nature of the VHT/lambda2 BCR, including the reactivity with ssDNA. Taken together, it is suggested that polyreactivity (including self-reactivity) of BCR is crucial in driving B cells to differentiate into the MZ phenotype.
- リンク情報
- ID情報
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- ISSN : 0022-1767
- ORCIDのPut Code : 28821038
- Web of Science ID : WOS:000226571300038