Nov, 2009
Novel reporter cell line to analyze cytokine-mediated expression regulation of c-myc gene
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
- Volume
- 108
- Number
- 5
- First page
- 438
- Last page
- 440
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1016/j.jbiosc.2009.04.022
- Publisher
- SOC BIOSCIENCE BIOENGINEERING JAPAN
Growth-promoting cytokines induce expression of nuclear proto-oncogenes that play critical roles in the regulation of cell proliferation. c-myc gene is one of those nuclear proto-oncogenes, whose regulation mechanisms of cytokine-mediated expression are not fully understood. Here, I generated a green fluorescent protein (GFP) reporter system that faithfully reflects interleukin-3 (IL-3)-induced c-myc gene expression. Flowcytometric analysis revealed cytokine-specific expression of reporter GFP. Kinetics of GFP mRNA expression was similar to that of endogenous c-myc mRNA. The reporter cell line will be a useful tool for studies of cell proliferation regulation through analysis of cytokine-induced c-myc gene expression. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.
- Link information
-
- DOI
- https://doi.org/10.1016/j.jbiosc.2009.04.022
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/19804871
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000273247700016&DestApp=WOS_CPL
- URL
- http://europepmc.org/abstract/med/19804871
- URL
- http://orcid.org/0000-0003-1296-4256
- ID information
-
- DOI : 10.1016/j.jbiosc.2009.04.022
- ISSN : 1389-1723
- ORCID - Put Code : 49884994
- Pubmed ID : 19804871
- Web of Science ID : WOS:000273247700016