2021年11月24日
Development of a Dengue Virus Serotype-Specific Non-Structural Protein 1 Capture Immunochromatography Method.
Sensors (Basel, Switzerland)
- 巻
- 21
- 号
- 23
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.3390/s21237809
Four serotypes of dengue virus (DENV), type 1 to 4 (DENV-1 to DENV-4), exhibit approximately 25-40% of the difference in the encoded amino acid residues of viral proteins. Reverse transcription of RNA extracted from specimens followed by PCR amplification is the current standard method of DENV serotype determination. However, since this method is time-consuming, rapid detection systems are desirable. We established several mouse monoclonal antibodies directed against DENV non-structural protein 1 and integrated them into rapid DENV detection systems. We successfully developed serotype-specific immunochromatography systems for all four DENV serotypes. Each system can detect 104 copies/mL in 15 min using laboratory and clinical isolates of DENV. No cross-reaction between DENV serotypes was observed in these DENV isolates. We also confirmed that there was no cross-reaction with chikungunya, Japanese encephalitis, Sindbis, and Zika viruses. Evaluation of these systems using serum from DENV-infected individuals indicated a serotype specificity of almost 100%. These assay systems could accelerate both DENV infection diagnosis and epidemiologic studies in DENV-endemic areas.
- リンク情報
-
- DOI
- https://doi.org/10.3390/s21237809
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/34883813
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8659457
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85119671479&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85119671479&origin=inward
- ID情報
-
- DOI : 10.3390/s21237809
- ISSN : 1424-8220
- PubMed ID : 34883813
- PubMed Central 記事ID : PMC8659457
- SCOPUS ID : 85119671479