2014年4月
Multiple BiP Genes of Arabidopsis thaliana are Required for Male Gametogenesis and Pollen Competitiveness
PLANT AND CELL PHYSIOLOGY
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- ,
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- 巻
- 55
- 号
- 4
- 開始ページ
- 801
- 終了ページ
- 810
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/pcp/pcu018
- 出版者・発行元
- OXFORD UNIV PRESS
Immunoglobulin-binding protein (BiP) is a molecular chaperone of the heat shock protein 70 (Hsp70) family. BiP is localized in the endoplasmic reticulum (ER) and plays key roles in protein translocation, protein folding and quality control in the ER. The genomes of flowering plants contain multiple BiP genes. Arabidopsis thaliana has three BiP genes. BIP1 and BIP2 are ubiquitously expressed. BIP3 encodes a less well conserved BiP paralog, and it is expressed only under ER stress conditions in the majority of organs. Here, we report that all BiP genes are expressed and functional in pollen and pollen tubes. Although the bip1 bip2 double mutation does not affect pollen viability, the bip1 bip2 bip3 triple mutation is lethal in pollen. This result indicates that lethality of the bip1 bip2 double mutation is rescued by BiP3 expression. A decrease in the copy number of the ubiquitously expressed BiP genes correlates well with a decrease in pollen tube growth, which leads to reduced fitness of mutant pollen during fertilization. Because an increased protein secretion activity is expected to increase the protein folding demand in the ER, the multiple BiP genes probably cooperate with each other to ensure ER homeostasis in cells with active secretion such as rapidly growing pollen tubes.
- リンク情報
- ID情報
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- DOI : 10.1093/pcp/pcu018
- ISSN : 0032-0781
- eISSN : 1471-9053
- PubMed ID : 24486762
- Web of Science ID : WOS:000334679500014