2010年
Protein-Protein Interaction Changes in an Archaeal Light-Signal Transduction
JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY
- ,
- ,
- 巻
- 2010
- 号
- 開始ページ
- Article ID 424760
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 書評論文,書評,文献紹介等
- DOI
- 10.1155/2010/424760
- 出版者・発行元
- HINDAWI PUBLISHING CORPORATION
Negative phototaxis in Natronomonas pharaonis is initiated by transient interaction changes between photoreceptor and transducer. pharaonis phoborhodopsin (ppR; also called pharaonis sensory rhodopsin II, psR-II) and the cognate transducer protein, pHtrII, form a tight 2 : 2 complex in the unphotolyzed state, and the interaction is somehow altered during the photocycle of ppR. We have studied the signal transduction mechanism in the ppR/pHtrII system by means of low-temperature Fourier-transform infrared (FTIR) spectroscopy. In the paper, spectral comparison in the absence and presence of pHtrII provided fruitful information in atomic details, where vibrational bands were identified by the use of isotope-labeling and site-directed mutagenesis. From these studies, we established the two pathways of light-signal conversion from the receptor to the transducer; (i) from Lys205 (retinal) of ppR to Asn74 of pHtrII through Thr204 and Tyr199, and (ii) from Lys205 of ppR to the cytoplasmic loop region of pHtrII that links Gly83.
- リンク情報
- ID情報
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- DOI : 10.1155/2010/424760
- ISSN : 1110-7243
- eISSN : 1110-7251
- Web of Science ID : WOS:000280880100001