2006年8月
Capture and release of DNA using aminosilane-modified bacterial magnetic particles for automated detection system of single nucleotide polymorphisms
BIOTECHNOLOGY AND BIOENGINEERING
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- 巻
- 94
- 号
- 5
- 開始ページ
- 862
- 終了ページ
- 868
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1002/bit.20904
- 出版者・発行元
- JOHN WILEY & SONS INC
Bacterial magnetic particles (BMPs) were modified with 3-[2-(2-aminoethylamino)-ethylaminol-propyltrimethoxysilane (AEEA) to produce a dense amine surface. Modification of BMPs in a toluene solution resulted in an increased amine yield, and approximately 11.3 x 10(4) surface amines were detected on a single particle. The modified BMPs were capable of efficient electrostatic capture of DNA. The maximum amount of DNA captured on 10 mu g of aminosilane-modified BMPs was 600 ng. A 10 mM phosphate buffer effectively released the captured DNA. This efficiency was dramatically enhanced by incubation at 80 degrees C and DNA recovery from aminosilane-modified BMPs approached 95%. DNA extraction from whole blood using these modified BMPs, followed by PCR, was successfully performed. Furthermore, automated single nucleotide polymorphism (SNP) detection of the aldehyde clehydrogenase 2 (ALDH2) was demonstrated. (c) 2006 Wiley Periodicals, Inc.
- リンク情報
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- DOI
- https://doi.org/10.1002/bit.20904
- CiNii Articles
- http://ci.nii.ac.jp/naid/80019062260
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/16523525
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000239046900005&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1002/bit.20904
- ISSN : 0006-3592
- CiNii Articles ID : 80019062260
- PubMed ID : 16523525
- Web of Science ID : WOS:000239046900005