Jan, 2006
The nitrodibenzofuran chromophore: a new caging group for ultra-efficient photolysis in living cells.
Nature Methods
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- Volume
- 3
- Number
- 1
- First page
- 35-40
- Last page
- 40
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1038/NMETH821
- Publisher
- NATURE PUBLISHING GROUP
Photochemical uncaging of bio-active molecules was introduced in 1977, but since then, there has been no substantial improvement in the properties of generic caging chromophores. We have developed a new chromophore, nitrodibenzofuran (NDBF) for ultra-efficient uncaging of second messengers inside cells. Photolysis of a NDBF derivative of EGTA (caged calcium) is about 16-160 times more efficient than photolysis of the most widely used caged compounds (the quantum yield of photolysis is 0.7 and the extinction coefficient is 18,400 M-1 cm(-1)). Ultraviolet (UV)-laser phototysis of NDBF-EGTA:Ca2+ rapidly released Ca2+ (rate of 20,000 s(-1)) and initiated contraction of skinned guinea pig cardiac muscle. NDBF-EGTA has a two-photon cross-section of similar to 0.6 GM and two-photon phototysis induced localized Ca2+-induced Ca2+ release from the sarcoplasmic recticulum of intact cardiac myocytes. Thus, the NDBF chromophore has great promise as a generic and photochemically efficient protecting group for both one- and two-photon uncaging in living cells.
- Link information
- ID information
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- DOI : 10.1038/NMETH821
- ISSN : 1548-7091
- Web of Science ID : WOS:000234528000015