2018年1月1日
Identification of microRNA that represses IRS-1 expression in liver
PLoS ONE
- 巻
- 13
- 号
- 1
- 開始ページ
- e0191553
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1371/journal.pone.0191553
- 出版者・発行元
- Public Library of Science
MicroRNAs (miRNAs) are short, non-coding RNAs that post-transcriptionally regulate gene expression and have been shown to participate in almost every cellular process. Several miRNAs have recently been implicated in glucose metabolism, but the roles of miRNAs in insulin-resistant conditions, such as obesity or type 2 diabetes, are largely unknown. Herein, we focused on miR-222, the expression of which was increased in the livers of high fat/high sucrose diet-fed mice injected with gold thioglucose (G+HFHSD). Overexpression of miR-222 in primary mouse hepatocytes attenuated Akt phosphorylation induced by insulin, indicating that miR-222 negatively regulates insulin signaling. As per in silico analysis, miR-222 potentially binds to the 30 untranslated region (30 UTR) of the IRS-1 gene, a key insulin signaling molecule. In fact, IRS-1 protein expression was decreased in the livers of G+HFHSD-fed mice. We further confirmed a direct interaction between miR-222 and the 30 UTR of IRS-1 via luciferase assays. Our findings suggest that up-regulation of miR-222 followed by reduction in IRS-1 expression may be a viable mechanism of insulin resistance in the liver.
- リンク情報
-
- DOI
- https://doi.org/10.1371/journal.pone.0191553
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/29364977
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85041130680&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85041130680&origin=inward
- ID情報
-
- DOI : 10.1371/journal.pone.0191553
- ISSN : 1932-6203
- eISSN : 1932-6203
- PubMed ID : 29364977
- SCOPUS ID : 85041130680