2014年11月
Whole-Body Imaging with Single-Cell Resolution by Tissue Decolorization
CELL
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- 巻
- 159
- 号
- 4
- 開始ページ
- 911
- 終了ページ
- 924
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.cell.2014.10.034
- 出版者・発行元
- CELL PRESS
The development of whole-body imaging at single-cell resolution enables system-level approaches to studying cellular circuits in organisms. Previous clearing methods focused on homogenizing mismatched refractive indices of individual tissues, enabling reductions in opacity but falling short of achieving transparency. Here, we show that an aminoalcohol decolorizes blood by efficiently eluting the heme chromophore from hemoglobin. Direct transcardial perfusion of an aminoalcohol-containing cocktail that we previously termed CUBIC coupled with a 10 day to 2 week clearing protocol decolorized and rendered nearly transparent almost all organs of adult mice as well as the entire body of infant and adult mice. This CUBIC-perfusion protocol enables rapid whole-body and whole-organ imaging at single-cell resolution by using light-sheet fluorescent microscopy. The CUBIC protocol is also applicable to 3D pathology, anatomy, and immunohistochemistry of various organs. These results suggest that whole-body imaging of colorless tissues at high resolution will contribute to organism-level systems biology.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.cell.2014.10.034
- ISSN : 0092-8674
- eISSN : 1097-4172
- Web of Science ID : WOS:000344522000021