論文

2020年7月

Raman image-activated cell sorting

NATURE COMMUNICATIONS
  • Nao Nitta
  • Takanori Iino
  • Akihiro Isozaki
  • Mai Yamagishi
  • Yasutaka Kitahama
  • Shinya Sakuma
  • Yuta Suzuki
  • Hiroshi Tezuka
  • Minoru Oikawa
  • Fumihito Arai
  • Takuya Asai
  • Dinghuan Deng
  • Hideya Fukuzawa
  • Misa Hase
  • Tomohisa Hasunuma
  • Takeshi Hayakawa
  • Kei Hiraki
  • Kotaro Hiramatsu
  • Yu Hoshino
  • Mary Inaba
  • Yuki Inoue
  • Takuro Ito
  • Masataka Kajikawa
  • Hiroshi Karakawa
  • Yusuke Kasai
  • Yuichi Kato
  • Hirofumi Kobayashi
  • Cheng Lei
  • Satoshi Matsusaka
  • Hideharu Mikami
  • Atsuhiro Nakagawa
  • Keiji Numata
  • Tadataka Ota
  • Takeichiro Sekiya
  • Kiyotaka Shiba
  • Yoshitaka Shirasaki
  • Nobutake Suzuki
  • Shunji Tanaka
  • Shunnosuke Ueno
  • Hiroshi Watarai
  • Takashi Yamano
  • Masayuki Yazawa
  • Yusuke Yonamine
  • Dino Di Carlo
  • Yoichiroh Hosokawa
  • Sotaro Uemura
  • Takeaki Sugimura
  • Yasuyuki Ozeki
  • Keisuke Goda
  • 全て表示

11
1
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41467-020-17285-3
出版者・発行元
NATURE PUBLISHING GROUP

The advent of image-activated cell sorting and imaging-based cell picking has advanced our knowledge and exploitation of biological systems in the last decade. Unfortunately, they generally rely on fluorescent labeling for cellular phenotyping, an indirect measure of the molecular landscape in the cell, which has critical limitations. Here we demonstrate Raman image-activated cell sorting by directly probing chemically specific intracellular molecular vibrations via ultrafast multicolor stimulated Raman scattering (SRS) microscopy for cellular phenotyping. Specifically, the technology enables real-time SRS-image-based sorting of single live cells with a throughput of up to similar to 100 events per second without the need for fluorescent labeling. To show the broad utility of the technology, we show its applicability to diverse cell types and sizes. The technology is highly versatile and holds promise for numerous applications that are previously difficult or undesirable with fluorescence-based technologies.

リンク情報
DOI
https://doi.org/10.1038/s41467-020-17285-3
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000548306600004&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/s41467-020-17285-3
  • ISSN : 2041-1723
  • Web of Science ID : WOS:000548306600004

エクスポート
BibTeX RIS