2011年2月
Stage-specific roles of fibulin-5 during oxidative stress-induced renal carcinogenesis in rats
FREE RADICAL RESEARCH
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- 巻
- 45
- 号
- 2
- 開始ページ
- 211
- 終了ページ
- 220
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.3109/10715762.2010.523702
- 出版者・発行元
- INFORMA HEALTHCARE
By using a rat model of renal cell carcinoma (RCC) induced by ferric nitrilotriacetate (Fe-NTA), this study performed genome-wide analysis to identify target genes during carcinogenesis. It screened for genes with decreased expression in RCCs, with simultaneous loss of heterozygosity, eventually to focus on the fibulin-5 (fbln5) gene. Oxidative damage via Fe-NTA markedly increased Fbln5 in the proximal tubules. RCCs presented lower levels of Fbln5. However, a fraction of RCCs presenting pulmonary metastasis revealed significantly higher levels of Fbln5 than those without metastasis, accompanied by immunopositivity of RCC cells and myofibroblast proliferation. Experiments revealed that RCC cell lines showed lower expression of fbln5 than its non-transformed counterpart NRK52E, but that fbln5 transfection to RCC cell lines changed neither proliferation nor migration/invasion. The data suggest that Fbln5 plays a role not only in the tissue repair and remodelling after renal tubular oxidative damage but also in RCC metastasis, presumably as a cytokine.
- リンク情報
- ID情報
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- DOI : 10.3109/10715762.2010.523702
- ISSN : 1071-5762
- PubMed ID : 20942562
- Web of Science ID : WOS:000287090300010