Ryo Kitahara

Profile Information

Affiliation: Professor, College of Pharmaceutical Sciences, Department of Pharmaceutical Sciences, Ritsumeikan University
: (Concurrent)Research Organization of Science and Technology, Research Center for Drug Discovery and Development Scieces

Degree: Ph.D. (Science)(Mar, 2002, Kobe University)

J-GLOBAL ID: 200901076796947327
researchmap Member ID: 6000017423

External link: http://www.ritsumei.ac.jp/pharmacy/kitahara/kitahara_lab.html

Research Interests

protein, pressure, structure, semi-stable conformer, conformational fluctuation

Research Areas

Research History

Apr, 2017 - Mar, 2018

Institute for Protein Research, Osaka University
Apr, 2011 - Mar, 2017

Ritsumeikan University, Colleges of Pharmaceutical Science
Apr, 2008 - Mar, 2011

Ritsumeikan University, Colleges of Pharmaceutical Science
Apr, 2008 - Mar, 2010

理化学研究所・客員研究員
Nov, 2009

Shiga University of Medical Science
Apr, 2005 - Mar, 2008

(独）理化学研究所訪問研究員
Apr, 2005 - Mar, 2008

（独）日本学術振興会特別研究員
Apr, 2002 - Mar, 2005

(独）理化学研究所基礎科学特別研究員

Education

Committee Memberships

Apr, 2017 - Mar, 2019

評議員, 日本核磁気共鳴学会
Apr, 2012 - Apr, 2014

「生物物理」編集委員, 日本生物物理学会
Apr, 2012 - Mar, 2014

日本核磁気共鳴学会評議員
2008

生物物理編集地区委員
2008

生物物理学会専門委員

Awards

Nov, 2006

生物物理学会若手奨励賞, 日本生物物理学会
Nov, 2005

ＮＭＲ討論会ポスター賞

Papers

Phase separation by biopolymers: Basics and applications.

Ryo Kitahara, Tomoshi Kameda

Biophysics and physicobiology, 19 e190028, 2022
Pressure-Jump Kinetics of Liquid–Liquid Phase Separation: Comparison of Two Different Condensed Phases of the RNA-Binding Protein, Fused in Sarcoma

Ryo Kitahara, Ryota Yamazaki, Fumika Ide, Shujie Li, Yutaro Shiramasa, Naoya Sasahara, Takuya Yoshizawa

Journal of the American Chemical Society, 143(47) 19697-19702, Dec 1, 2021
Pressure and Temperature Phase Diagram for Liquid-Liquid Phase Separation of the RNA-Binding Protein Fused in Sarcoma

Shujie Li, Takuya Yoshizawa, Ryota Yamazaki, Ayano Fujiwara, Tomoshi Kameda, Ryo Kitahara

JOURNAL OF PHYSICAL CHEMISTRY B, 125(25) 6821-6829, Jul, 2021
Dynamic aspects of pressure and temperature-stabilized intermediates of outer surface protein A.

Takuro Wakamoto, Soichiro Kitazawa, Tomoshi Kameda, Ryo Kitahara

Proteins, 88(11) 1423-1433, Nov, 2020
Volumetric Properties for the Binding of 1,4-Dioxane to Amide Naphthotubes in Water.

Shujie Li, Huan Yao, Tomoshi Kameda, Wei Jiang, Ryo Kitahara

The journal of physical chemistry. B, 124(41) 9175-9181, Oct 15, 2020
How internal cavities destabilize a protein

Mengjun Xue, Takuro Wakamoto, Camilla Kejlberg, Yuichi Yoshimura, Tania Acquits Nielsen, Michael Wulff Risor, Kristian Weise Sanggaard, Ryo Kitahara, Frans AA Mulder

Proc. Natl. Acad. Sci. USA, 116(42) 21031-21036, Oct, 2019
Paramagnetic relaation enhancement-assisted structural characterization of a partially disordered conformation of ubiquitin

Takuro Wakamoto, Teppei Iseya, Soichiro Kitazawa, Nicola J. Baxter, Mike P. Williamson, Ryo Kitahara

Protein Science, 28(11) 1993-2003-2003, Oct, 2019
Pressure accelerates the circadian clock of cyanobacteria.

Ryo Kitahara, Katsuaki Oyama, Takahiro Kawamura, Keita Mitsuhashi, Soichiro Kitazawa, Kazuhiro Yasunaga, Natsuno Sagara, Megumi Fujimoto, Kazuki Terauchi

Scientific reports, 9(1) 12395-12395, Aug 27, 2019
Gene delivery to cone photoreceptors by subretinal injection of rAAV2/6 in the mouse retina.

Tesshu Hori, Masashi Fukutome, Chiseto Maejima, Hiroki Matsushima, Kensuke Kobayashi, Soichiro Kitazawa, Ryo Kitahara, Katsunori Kitano, Kenta Kobayashi, Satoru Moritoh …

Biochemical and biophysical research communications, 515(1) 222-227, Jul 12, 2019
Rational design using sequence information only produces a peptide that binds to the intrinsically disordered region of p53.

Kiyoto Kamagata, Eriko Mano, Yuji Itoh, Takuro Wakamoto, Ryo Kitahara, Saori Kanbayashi, Hiroto Takahashi, Agato Murata, Tomoshi Kameda

Scientific reports, 9(1) 8584-8584, Jun 28, 2019
Characterization of low-lying exited states of proteins by high-pressure NMR spectroscopy

Mike P. Williamson, Ryo Kitahara

BBA Proteins and Proteomics, 1867 350-358, Mar, 2019
Water-protein interactions coupled with protein conformational transition

Soichiro Kitazawa, Yu Aoshima, Takuro Wakamoto, Ryo Kitahara

Biophysical Journal, 115 981-987, Sep, 2018
How and why internal cavities destabilize proteins

Mulder Frans A. A, Xue Mengjun, Yoshimura Yuichi, Kitahara Ryo

FASEB JOURNAL, 32(1), Apr, 2018 Peer-reviewed
Nuclear magnetic resonance-based determination of dioxygen binding sites in protein cavities

Ryo Kitahara, Shun Sakuraba, Tomoshi Kameda, Sanshiro Okuda, Mengjun Xue, Frans A.A. Mulder

Protein Science, 27(3) 769-779, Mar 1, 2018
NMR法で観る圧力によるタンパク質の構造変化

北原亮

応用糖質科学, 7(4) 204-209, Dec, 2017
Interactions Controlling the Slow Dynamic Conformational Motions of Ubiquitin

Soichiro Kitazawa, Maho Yagi-Utsumi, Koichi Kato, Ryo Kitahara

MOLECULES, 22(9) 1414, Sep, 2017
Analysis of O-2-binding Sites in Proteins Using Gas-Pressure NMR Spectroscopy: Outer Surface Protein A

Takahiro Kawamura, Takuro Wakamoto, Soichiro Kitazawa, Shun Sakuraba, Tomoshi Kameda, Ryo Kitahara

BIOPHYSICAL JOURNAL, 112(9) 1820-1828, May, 2017
High-pressure NMR approaches on proteins

Ryo Kitahara

Review of High Pressure Science and Technology/Koatsuryoku No Kagaku To Gijutsu, 27(1) 13-17, 2017
Aberrant increase of NMR signal in hydrogen exchange experiments. Observation and explanation

Mengjun Xue, Ryo Kitahara, Yuichi Yoshimura, Frans A. A. Mulder

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 478(3) 1185-1188, Sep, 2016
Modeling N-15 NMR chemical shift changes in protein backbone with pressure

Giovanni La Penna, Yoshiharu Mori, Ryo Kitahara, Kazuyuki Akasaka, Yuko Okamoto

JOURNAL OF CHEMICAL PHYSICS, 145(8) 085104, Aug, 2016

Misc.

Rational Design of Peptide Targeting Intrinsically Disordered Protein p53-Regulation of Function and Phase Separation

Kiyoto Kamagata, Ryo Kitahara, Tomoshi Kameda

BIOPHYSICAL JOURNAL, 118(3) 492A-493A, Feb, 2020
NMR explorations of biomolecular systems with rapid conformational exchanges

M. Yagi-Utsumi, T. Yamaguchi, R. Kitahara, K. Kato

Molecular Science of Fluctuations toward Biological Functions (M. Terazima, M. Kataoka, R. Ueoka, and Y. Okamoto eds), Springer, 87-103, 2016
ガス圧NMR法によるタンパク質内部キャビティーと気体分子の相互作用研究

北原亮, 吉村優一, XUE Mengjun, 亀田倫史, MULDER Frans A.A

高圧討論会講演要旨集, 56th 63, Oct 27, 2015
Design Strategy for High Free-Energy States of Proteins Based on High-Pressure NMR Study: Alternatively Folded Conformation of Ubiquitin

Ryo Kitahara, Soichiro Kitazawa, Maho Yagi-Utsumi, Akemi Ido, Makoto Urushitani, Kenji Sugase, Koichi Kato

BIOPHYSICAL JOURNAL, 102(3) 25A-25A, Jan, 2012
Analysis of fluctuating protein structure - Approach from variable pressure NMR

Ryo Kitahara, Kazuyuki Akasaka

Review of High Pressure Science and Technology/Koatsuryoku No Kagaku To Gijutsu, 17(1) 32-41, 2007
2P052 Variable pressure NMR revealed a conserved exited state conformer between ubiquitin and ubiquitin-like protein NEDD8

Kitahara R., Sakata E., Kasuya T., Yamaguchi Y., Kato K., Tanaka K., Yokoyama S., Akasaka K.

Seibutsu Butsuri, 45(1) S132, Oct 19, 2005
High-pressure NMR spectroscopy for characterizing folding intermediates and denatured states of proteins

YO Kamatari, R Kitahara, H Yamada, S Yokoyama, K Akasaka

METHODS, 34(1) 133-143, Sep, 2004
High pressure NMR study of dihydrofolate reductase from a deep-sea bacterium Moritella profunda

K Hata, R Kono, M Fujisawa, R Kitahara, YO Kamatari, K Akaska, Y Xu

CELLULAR AND MOLECULAR BIOLOGY, 50(4) 311-316, Jun, 2004
Close identity of a pressure-stabilized intermediate with a kinetic intermediate in protein folding

R Kitahara, K Akasaka

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 100(6) 3167-3172, Mar, 2003
High-pressure H-1 NMR study of pressure-induced structural changes in the heme environments of metcyanomyoglobins

R Kitahara, M Kato, Y Taniguchi

PROTEIN SCIENCE, 12(2) 207-217, Feb, 2003
Equilibrium and pressure-jump relaxation studies of the conformational transitions of P13(MTCP1)

R Kitahara, C Royer, H Yamada, M Boyer, JL Saldana, K Akasaka, C Roumestand

JOURNAL OF MOLECULAR BIOLOGY, 320(3) 609-628, Jul, 2002
High pressure NMR reveals that apomyoglobin is an equilibrium mixture from the native to the unfolded

R Kitahara, H Yamada, K Akasaka, PE Wright

JOURNAL OF MOLECULAR BIOLOGY, 320(2) 311-319, Jul, 2002
Two folded conformers of ubiquitin revealed by high-pressure NMR

R Kitahara, H Yamada, K Akasaka

BIOCHEMISTRY, 40(45) 13556-13563, Nov, 2001
High pressure NMR reveals active-site hinge motion of folate-bound Escherichia coli dihydrofolate reductase

R Kitahara, S Sareth, H Yamada, E Ohmae, K Gekko, K Akasaka

BIOCHEMISTRY, 39(42) 12789-12795, Oct, 2000

Books and Other Publications

Molecular Science of Fluctuations toward Biological Functions [NMR explorations of biomolecular systems with rapid conformational exchanges]

M. Terazima, M. Kataoka, R. Ueoka, Y. Okamoto eds, Yagi-Utsumi, M, Yamaguchi, T, Kitahara, R, Kato, K

Springer, Mar, 2016 (ISBN: 9784431558385)
High Pressure Bioscience [High pressure NMR discloses functional sub-states of ubiquitin and ubiquitin-like proteins]

Akasaka K, Matsuki H. Eds, Ryo Kitahara

Springer, Aug, 2015 (ISBN: 9789401799171)
「揺らぎ・ダイナミクスと生体機能」寺嶋正秀編

化学同人, Sep, 2013 (ISBN: 9784759815108)
広がるＮＭＲの世界(朝倉哲郎編) 「高圧力NMRで見る蛋白質の高エネルギー構造の世界」の１トピックス

朝倉哲郎

コロナ社, Apr, 2011

Presentations

152

Water-protein interactions coupled with protein conformational transition

第５７回日本生物物理学会年会, Sep 26, 2019
Rational design of peptide targeting intrinsically disordered protein p53-reguation of function and phase-phase separation-

第５７回日本生物物理学会年会, Sep 25, 2019
Pressure accelerates the circadian clock of cyanobacteria

第５７回日本生物物理学会年会, Sep 24, 2019
Current trend of phase separation

第５７回日本生物物理学会年会, Sep 24, 2019
High-pressure NMR study of the locally disordered conformation of outer surface protein A (OspA)

8th Asia-Pacific NMR Symposium 2019, Jul 5, 2019
High-pressure NMR spectroscopy as a tool for studying protein dynamics

8th Asia-Pacific NMR Symposium 2019, Jul 4, 2019
シアノバクテリアの生物時計は高圧力下でどうなる？

第１９回蛋白質科学会年会, Jun 25, 2019
High-Pressure CLEANX-PM NMR法によるUbiquitinの構造ゆらぎに伴う水和環境の変化

第１９回蛋白質科学会年会, Jun 25, 2019
常磁性緩和促進法によるOuter surface protein Aの局所変性状態の立体構造解析

第１９回蛋白質科学会年会, Jun 24, 2019
Pressure accelerates the circadian clock of cyanobacteria

10th International conference on high pressure bioscience and biotechnology, Sep 19, 2018
High-pressure NMR study of the locally disordered conformation of outer surface protein A (OspA)

10th International conference on high pressure bioscience and biotechnology, Sep 19, 2018
リン酸化ユビキチンの２つのコンフォメーションの構造変化

第５７回NMR討論会, Sep 18, 2018
High-pressure NMR spectroscopy: Tools for studying protein dynamics

ICMRBS2018, Aug 21, 2018
High-pressure NMR reveals water-protein interactions coupled with protein conformational transition

ICMRBS2018, Aug 20, 2018
ライム病に関わるボレリア菌の表層蛋白質Outer surface protein A (OspA)の変性中間体の立体構造解析

第６５回日本生化学会近畿支部例会, May 26, 2018
高圧力NMR法による蛋白質の変性中間体の立体構造解析：ライム病に関わるボレリア菌Outer surface protein A(OspA)

日本薬学会第１３８会年会, Mar 27, 2018
ガス圧NMR法によるタンパク質における酸素結合部位の決定

第５６回NMR討論会, Nov 14, 2017
高圧力NMR法によるユビキチンの局所変性状態の立体構造決定

第５６回NMR討論会, Nov 14, 2017
リン酸化ユビキチンの２つのコンフォメーションの立体構造決定と圧力効果

第５６回NMR討論会, Nov 14, 2017
ガス圧NMR法によるタンパク質と溶存酸素の相互作用解析

日本分光学会ＮＭＲ分光部会講習会ーNMRで見る生体分子の運動・平衡状態・活性化状態ー, Oct 6, 2017

Teaching Experience

Professional Memberships

Works

特別講演

Jan, 2007 Other
seminar: A new paradigm of protein structures

Feb, 2006 Other
seminar:Exploring the entire conformational space of proteins by high pressure NMR

Jul, 2004 Other

Research Projects

冬眠する細胞が持つ圧力耐性－新たな網膜疾患治療戦略への展開

科学研究費助成事業, 挑戦的研究(萌芽), 日本学術振興会, Jun, 2023 - Mar, 2026

小池千恵子, 前田亜希子, 北原亮, 高橋政代, 川村晃久
分子夾雑と生物時計

科学研究費助成事業, 新学術領域研究(研究領域提案型), 日本学術振興会, Apr, 2020 - Mar, 2022

北原亮
Pressure effects on the cyanobacterial circadian clock

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2019 - Mar, 2022

Kitahara Ryo
Research on the molecular basis of conformational dynamics of oncogene product Ras for application to the development of its specific inhibitors

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2014 - Mar, 2017

shima fumi, KITAHARA Ryou
High-energy conformations of proteins as a new target of structural biology

Grants-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (B), Japan Society for the Promotion of Science, Apr, 2013 - Mar, 2016

Ryo Kitahara
Identification of pathogenic domain in TDP-43 for ALS toward the antibody medicine

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2011 - Mar, 2014

URUSHITANI Makoto, KITAHARA Ryo, ITO Hidefumi
高エネルギー構造の解明による蛋白質構造揺らぎの可視化

科学研究費助成事業, 新学術領域研究(研究領域提案型), 日本学術振興会, Apr, 2011 - Mar, 2013

北原亮
High pressure NMR study for conformational fluctuation of polyubiquitin

Grants-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (B), Japan Society for the Promotion of Science, 2010 - 2011

KITAHARA Ryo
A novel approach to ALS pathogenesis by investigating conformational equilibrium of TDP-43

Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, Japan Society for the Promotion of Science, 2010 - 2011

URUSHITANI Makoto, KITAHARA Ryo
圧力・温度摂動ＮＭＲ法による蛋白質の構造揺らぎ研究

財団法人サントリー生物有機科学研究所研究奨励金, 2009 - 2011
高圧力NMR法による蛋白質の準安定状態にもとづいた構造・機能相関

科学研究費助成事業, 新学術領域研究(研究領域提案型), 日本学術振興会, 2009 - 2010

北原亮
高圧ＮＭＲ法による広い構造空間における蛋白質の構造解析

2002 - 2005

Industrial Property Rights

6332723 TDP-43の凝集体が蓄積する疾患を治療及び／又は予防するための化合物のスクリーニング方法

Profile Information

Research Interests

Research Areas

Research History

Education

Committee Memberships

Awards

Papers

Misc.

Books and Other Publications

Presentations

Teaching Experience

Professional Memberships

Works

Research Projects

Industrial Property Rights

Menu

Coauthors