2010年7月
Identification of novel ribonucleo-protein complexes from the brain-specific snoRNA MBII-52
RNA
- ,
- ,
- ,
- ,
- ,
- 巻
- 16
- 号
- 7
- 開始ページ
- 1293
- 終了ページ
- 1300
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1261/rna.2109710
- 出版者・発行元
- COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
Small nucleolar RNAs (snoRNAs) guide nucleotide modifications within ribosomal RNAs or spliceosomal RNAs by base-pairing to complementary regions within their RNA targets. The brain-specific snoRNA MBII-52 lacks such a complementarity to rRNAs or snRNAs, but instead has been reported to target the serotonin receptor 2C pre-mRNA, thereby regulating pre-mRNA editing and/or alternative splicing. To understand how the MBII-52 snoRNA might be involved in these regulatory processes, we isolated the MBII-52 snoRNP from total mouse brain by an antisense RNA affinity purification approach. Surprisingly, by mass spectrometry we identified 17 novel candidates for MBII-52 snoRNA binding proteins, which previously had not been reported to be associated with canonical snoRNAs. Among these, Nucleolin and ELAVL1 proteins were confirmed to independently and directly interact with the MBII-52 snoRNA by coimmunoprecipitation. Our findings suggest that the MBII-52 snoRNA assembles into novel RNA-protein complexes, distinct from canonical snoRNPs. Copyright © 2010 RNA Society.
- リンク情報
-
- DOI
- https://doi.org/10.1261/rna.2109710
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/20484469
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000279048800001&DestApp=WOS_CPL
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77953640328&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=77953640328&origin=inward
- ID情報
-
- DOI : 10.1261/rna.2109710
- ISSN : 1355-8382
- eISSN : 1469-9001
- ORCIDのPut Code : 104821645
- PubMed ID : 20484469
- SCOPUS ID : 77953640328
- Web of Science ID : WOS:000279048800001