1999年5月15日
Effect of the removal of extracellular Ca2+ on the response of cytosolic concentrations of Ca2+ to ouabain in carotid body glomus cells of adult rabbits
Brain Research
- 巻
- 828
- 号
- 1-2
- 開始ページ
- 193
- 終了ページ
- 196
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/S0006-8993(99)01359-1
Effect of the removal of extracellular Ca2+ on the response of cytosolic concentrations of Ca2+ ([Ca2+](i)) to ouabain, an Na+/K+ exchanger antagonist, was examined in clusters of cultured carotid body glomus cells of adult rabbits using fura-2AM and microfluorometry. Application of ouabain (10 mM) induced a sustained increase in [Ca2+](i) (mean ± S.E.M.
38 ± 5% increase, n = 16) in 55% of tested cells (n = 29). The ouabain-induced [Ca2+](i) increase was abolished by the removal of extracellular Na+. D600 (50 μM), an L-type voltage-gated Ca2+ channel antagonist, inhibited the [Ca2+](i) increase by 57 ± 7% (n = 4). Removal of extracellular Ca2+ eliminated the [Ca2+](i) increase, but subsequent washing out of ouabain in Ca2+-free solution produced a rise in [Ca2+](i) (62 ± 8% increase, n = 6, P<
0.05), referred to as a [Ca2+](i) rise after Ca2+-free/ouabain. The magnitude of the [Ca2+](i) rise was larger than that of ouabain-induced [Ca2+](i) increase. D600 (5 μM) inhibited the [Ca2+](i) rise after Ca2+-free/ouabain by 83 ± 10% (n = 4). These results suggest that ouabain-induced [Ca2+](i) increase was due to Ca2+ entry involving L-type Ca2+ channels which could be activated by cytosolic Na+ accumulation. Ca2+ removal might modify the [Ca2+](i) response, resulting in the occurrence of a rise in [Ca2+](i) after Ca2+- free/ouabain which mostly involved L-type Ca2+ channels.
38 ± 5% increase, n = 16) in 55% of tested cells (n = 29). The ouabain-induced [Ca2+](i) increase was abolished by the removal of extracellular Na+. D600 (50 μM), an L-type voltage-gated Ca2+ channel antagonist, inhibited the [Ca2+](i) increase by 57 ± 7% (n = 4). Removal of extracellular Ca2+ eliminated the [Ca2+](i) increase, but subsequent washing out of ouabain in Ca2+-free solution produced a rise in [Ca2+](i) (62 ± 8% increase, n = 6, P<
0.05), referred to as a [Ca2+](i) rise after Ca2+-free/ouabain. The magnitude of the [Ca2+](i) rise was larger than that of ouabain-induced [Ca2+](i) increase. D600 (5 μM) inhibited the [Ca2+](i) rise after Ca2+-free/ouabain by 83 ± 10% (n = 4). These results suggest that ouabain-induced [Ca2+](i) increase was due to Ca2+ entry involving L-type Ca2+ channels which could be activated by cytosolic Na+ accumulation. Ca2+ removal might modify the [Ca2+](i) response, resulting in the occurrence of a rise in [Ca2+](i) after Ca2+- free/ouabain which mostly involved L-type Ca2+ channels.
- リンク情報
- ID情報
-
- DOI : 10.1016/S0006-8993(99)01359-1
- ISSN : 0006-8993
- identifiers.cinii_nr_id : 9000239248799
- PubMed ID : 10320743
- SCOPUS ID : 0033562475