2003年9月
Isolation of lectins by affinity chromatography with porcine plasma proteins immobilized on agarose
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
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- 巻
- 67
- 号
- 9
- 開始ページ
- 2051
- 終了ページ
- 2054
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1271/bbb.67.2051
- 出版者・発行元
- TAYLOR & FRANCIS LTD
To develop a convenient method to isolate lectins, we prepared an affinity gel by coupling plasma proteins with agarose beads under conditions where the pH did not exceed 7.5. The validity of the use of this affinity gel in combination with elution using a hapten saccharide was confirmed by isolation of concanavalin A from Jack bean meal. Successful application of the method was demonstrated by isolation of two novel vegetable lectins from udo (Aralia cordate) and wasabi (Wasabia japonica). The method would be useful to isolate new lectins from various sources including plant and animal tissues.
- リンク情報
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- DOI
- https://doi.org/10.1271/bbb.67.2051
- CiNii Articles
- http://ci.nii.ac.jp/naid/110002694355
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/14520004
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000185727700038&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1271/bbb.67.2051
- ISSN : 0916-8451
- eISSN : 1347-6947
- CiNii Articles ID : 110002694355
- PubMed ID : 14520004
- Web of Science ID : WOS:000185727700038