論文

査読有り
2014年8月

Identification and expression of troponin T, a new marker on the surface of cultured tumor endothelial cells by aptamer ligand

CANCER MEDICINE
  • Mst. Naznin Ara
  • ,
  • Mamoru Hyodo
  • ,
  • Noritaka Ohga
  • ,
  • Kosuke Akiyama
  • ,
  • Kyoko Hida
  • ,
  • Yasuhiro Hida
  • ,
  • Nobuo Shinohara
  • ,
  • Hideyoshi Harashima

3
4
開始ページ
825
終了ページ
834
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/cam4.260
出版者・発行元
WILEY

The identification of a specific biomarker involves the development of new clinical diagnostic tools, and an in-depth understanding of the disease at the molecular level. When new blood vessels form in tumor cells, endothelial cell production is induced, a process that plays a key role in disease progression and metastasis to distinct organs for solid tumor types. The present study reports on the identification of a new biomarker on primary cultured mouse tumor endothelial cells (mTECs) using our recently developed high-affinity DNA aptamer AraHH001 (K-d = 43 nmol/L) assisted proteomics approach. We applied a strategy involving aptamer-facilitated biomarker discovery. Biotin-tagged AraHH001 was incubated with lysates of mTECs and the aptamer-proteins were then conjugated with streptavidin magnetic beads. Finally, the bound proteins were separated by sodiumdodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) with silver staining. We identified troponin T via matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry, the molecular target of aptamer AraHH001, and its presence was confirmed by measuring mRNA, protein levels, western blot, immunostaining, a gel shift assay of AraHH001 with troponin T. We first report here on the discovery of troponin T on mTECs, a promising and interesting diagnostic tool in the development of antiangiogenic therapy techniques the involves the targeting of the tumor vasculature.

Web of Science ® 被引用回数 : 13

リンク情報
DOI
https://doi.org/10.1002/cam4.260
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24810801
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000348222300008&DestApp=WOS_CPL

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