2020年5月
Characterization of an intermediate filament protein from the platyhelminth, Dugesia japonica
Protein and Peptide Letters
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- 巻
- 27
- 号
- 5
- 開始ページ
- 432
- 終了ページ
- 446
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.2174/0929866526666191025102902
Background: Intermediate filaments (IFs) are major constituents of the cytoskeletal systems in animal cells. Objective: To gain insights into the structure-function relationship of invertebrate cytoplasmic IF proteins, we characterized an IF protein from the platyhelminth, Dugesia japonica, termed Dif-1. Methods: cDNA cloning, in situ hybridization, immunohistochemical analysis, and IF assembly experiments in vitro using recombinant Dif-1, were performed for protein characterization. Results: The structure deduced from the cDNA sequence showed that Djf-1 comprises 568 amino acids and has a tripartite domain structure (N-terminal head, central rod, and C-terminal tail) that is characteristic of IF proteins. Similar to nuclear IF lamins, Djf-1 contains an extra 42 residues in the coil 1b subdomain of the rod domain that is absent from vertebrate cytoplasmic IF proteins and a nuclear lamin-homology segment of approximately 105 residues in the tail domain; however, it contains no nuclear localization signal. In situ hybridization analysis showed that Djf-1 mRNA is specifically expressed in cells located within the marginal region encircling the worm body. Immunohistochemical analysis showed that Djf-1 protein forms cytoplasmic IFs located close to the microvilli of the cells. In vitro IF assembly experiments using recombinant proteins showed that Djf-1 alone polymerizes into IFs. Deletion of the extra 42 residues in the coil 1b subdomain resulted in the failure of IF formation. Conclusion: Together with data from other histological studies, our results suggest that Djf-1 is expressed specifically in anchor cells within the glandular adhesive organs of the worm and that Djf-1 IFs may play a role in protecting the cells from mechanical stress.
- リンク情報
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- DOI
- https://doi.org/10.2174/0929866526666191025102902
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/31652112
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85084721069&origin=inward
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85084721069&origin=inward
- ID情報
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- DOI : 10.2174/0929866526666191025102902
- ISSN : 0929-8665
- eISSN : 1875-5305
- PubMed ID : 31652112
- SCOPUS ID : 85084721069