1999年5月
Morphine prevents peroxynitrite-induced death of human neuroblastoma SH-SY5Y cells through a direct scavenging action
EUROPEAN JOURNAL OF PHARMACOLOGY
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- 巻
- 372
- 号
- 3
- 開始ページ
- 319
- 終了ページ
- 324
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/S0014-2999(99)00206-X
- 出版者・発行元
- ELSEVIER SCIENCE BV
N-Ethyl-2-(1-ethyl-2-hydroxy-2-nitrosohydrazino)-ethanamine (NOC12), a nitric oxide donor, 3-morpholinosydnonimine (SIN-1), a generator of peroxynitrite (ONOO-), and peroxynitrite induced cell death accompanied by DNA fragmentation in human neuroblastoma SH-SY5Y cell cultures. Morphine prevented the cell death induced by SIN-1 or peroxynitrite, but not that induced by NOC12. The protective effect of morphine was concentration-dependent (10-100 mu M), but was not antagonized by naloxone. The selective ligands for opioid receptor subtypes, [D-Ala(2), N-Me-Phe(4), Gly-ol(5)]enkephalin (DAMGO, mu-opioid receptor agonist), [D-Pen(2,5)]enkephalin (DPDPE, delta-opioid receptor agonist) and trans-(+/-)-3,4-dichloro-N-methyl-N-(2-[1-pyrrolidinyl]-cyclohexyl)benzeneacetamide (U-50488, kappa-opioid receptor agonist) even at the concentration of 100 mu M did not prevent the cell death induced by SIN-1. From measurement of the absorbance spectrum of peroxynitrite, the decomposition of peroxynitrite in 0.25 M potassium phosphate buffer (pH 7.4) was Very rapid and complete within seconds. However, the absorbance was very stable in the presence of morphine. In addition, morphine inhibited peroxynitrite-induced nitration of tyrosine in a concentration-dependent manner. These results indicate that morphine rapidly reacts with peroxynitrite. The present study showed that morphine prevented peroxynitrite-induced cell death through its direct scavenging action, suggesting that morphine can protect cells against damage caused by peroxynitrite. (C) 1999 Elsevier Science B.V. All rights reserved.
- リンク情報
-
- DOI
- https://doi.org/10.1016/S0014-2999(99)00206-X
- CiNii Articles
- http://ci.nii.ac.jp/naid/10011536941
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/10395028
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000080762500016&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1016/S0014-2999(99)00206-X
- ISSN : 0014-2999
- CiNii Articles ID : 10011536941
- PubMed ID : 10395028
- Web of Science ID : WOS:000080762500016