2011年1月
Connexon-mediated cell adhesion drives microtissue self-assembly
FASEB JOURNAL
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- 巻
- 25
- 号
- 1
- 開始ページ
- 255
- 終了ページ
- 264
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1096/fj.10-155291
- 出版者・発行元
- FEDERATION AMER SOC EXP BIOL
Microtissue self-assembly is thought to be driven primarily by cadherins, while connexons have been examined mainly in intercellular coupling. We investigated whether connexon 43 (Cx43)-mediated cell adhesion modulates self-assembly of human KGN granulosa cells, normal human fibroblasts (NHFs), and MCF-7 breast cancer cells seeded into nonadhesive agarose gels. We found that treatment with anti-Cx43 E2 (112 mu g/ml), which suppresses Cx43 docking, significantly inhibited the kinetics of KGN and NHF self-assembly compared to the preimmune sera control (41.1 +/- 4.5 and 24.5 +/- 10.4% at 8 h, respectively). Likewise, gap junction inhibitor carbenoxolone also inhibited self-assembly of KGN, NHF, and MCF-7 cells in a dose-dependent manner that was specific to cell type. In contrast, Gap26 connexin mimetic peptide, which inhibits channel permeability but not docking, accelerated self-assembly of KGN and NHF microtissues. Experiments using selective enzymatic digestion of cell adhesion molecules and neutralizing N-cadherin antibodies further showed that self-assembly was comparably disrupted by inhibiting connexin-and cadherin-mediated adhesion. These findings demonstrate that connexon-mediated cell adhesion and intercellular communication differentially influence microtissue self-assembly, and that their contributions are comparable to those of cadherins.-Bao, B., Jiang, J., Yanase, T., Nishi, Y., Morgan, J. R. Connexon-mediated cell adhesion drives microtissue self-assembly. FASEB J. 25, 255-264 (2011). www.fasebj.org
- リンク情報
- ID情報
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- DOI : 10.1096/fj.10-155291
- ISSN : 0892-6638
- Web of Science ID : WOS:000285869500023