論文

2018年2月6日

In vivo evaluation of cerebral hemodynamics and tissue morphology in rats during changing fraction of inspired oxygen based on spectrocolorimetric imaging technique

International Journal of Molecular Sciences
  • Afrina Mustari
  • ,
  • Takuya Kanie
  • ,
  • Satoko Kawauchi
  • ,
  • Shunichi Sato
  • ,
  • Manabu Sato
  • ,
  • Yasuaki Kokubo
  • ,
  • Izumi Nishidate

19
2
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3390/ijms19020491
出版者・発行元
MDPI AG

During surgical treatment for cerebrovascular diseases, cortical hemodynamics are often controlled by bypass graft surgery, temporary occlusion of arteries, and surgical removal of veins. Since the brain is vulnerable to hypoxemia and ischemia, interruption of cerebral blood flow reduces the oxygen supply to tissues and induces irreversible damage to cells and tissues. Monitoring of cerebral hemodynamics and alteration of cellular structure during neurosurgery is thus crucial. Sequential recordings of red-green-blue (RGB) images of in vivo exposed rat brains were made during hyperoxia, normoxia, hypoxia, and anoxia. Monte Carlo simulation of light transport in brain tissue was used to specify relationships among RGB-values and oxygenated hemoglobin concentration (CHbO), deoxygenated hemoglobin concentration (CHbR), total hemoglobin concentration (CHbT), hemoglobin oxygen saturation (StO2), and scattering power b. Temporal courses of CHbO, CHbR, CHbT, and StO2 indicated physiological responses to reduced oxygen delivery to cerebral tissue. A rapid decrease in light scattering power b was observed after respiratory arrest, similar to the negative deflection of the extracellular direct current (DC) potential in so-called anoxic depolarization. These results suggest the potential of this method for evaluating pathophysiological conditions and loss of tissue viability.

リンク情報
DOI
https://doi.org/10.3390/ijms19020491
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29415505

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