Apr, 2015 - Mar, 2017
A development of gene delivery system that enable to making early diagnosis and inhibiting metastasis.
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B) Grant-in-Aid for Young Scientists (B)
- Grant number
- 15K20540
- Japan Grant Number (JGN)
- JP15K20540
- Grant amount
-
- (Total)
- 3,900,000 Japanese Yen
- (Direct funding)
- 3,000,000 Japanese Yen
- (Indirect funding)
- 900,000 Japanese Yen
1. We established a lymph node metastatic subline from a highly metastatic AdCC cell line. DNA microarray hybridization and scanning were performed to compare RNA expression in lymph node metastatic cells with highly metastaticcells. The expression level of NNMT (Nicotinamide N-methyltransferase ) was up-regulated by 2000 times in lymph node metastatic cells. NNMT is known as a tumorigenesis agent. It was suggests that NNMT is involved in the metastasis in the AdCCs.
2.We performed miRNA microarray to identify differential miRNAs in serum pools of 6 OSCC patients and 6 health control. Then, we analyzed expression levels of miRNA in each serum sample of OSCC patients and control by real time PCR. MiR-183 showed significant over expression in patient group and was concerned with metastasis.
2.We performed miRNA microarray to identify differential miRNAs in serum pools of 6 OSCC patients and 6 health control. Then, we analyzed expression levels of miRNA in each serum sample of OSCC patients and control by real time PCR. MiR-183 showed significant over expression in patient group and was concerned with metastasis.
- Link information
- ID information
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- Grant number : 15K20540
- Japan Grant Number (JGN) : JP15K20540