2019年12月
Nuclear Accumulation of β-Catenin in Cancer Stem Cell Radioresistance and Stemness in Human Colon Cancer.
Anticancer research
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- 巻
- 39
- 号
- 12
- 開始ページ
- 6575
- 終了ページ
- 6583
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.21873/anticanres.13873
BACKGROUND/AIM: The aim of this study was to examine whether the Wnt/β-catenin signal activation is a cause of radioresistance in colon cancer by assessing the β-catenin localization and its correlation with cancer stem cells (CSCs). MATERIALS AND METHODS: The nuclear levels of β-catenin, the hallmark of Wnt activation, were analyzed in HCT116 and SW480 cells by immunohistochemistry, before and after irradiation. Further, we assessed CSC populations by staining for aldehyde dehydrogenase-1 (ALDH1) and CD44. RESULTS: β-catenin was localized predominantly in the nucleus and plasma membrane in SW480 and HCT116 cells, respectively. Compared to HCT116 cells, SW480 cells displayed higher Wnt activation. At 24 h after irradiation, most of the DSBs in SW480 cells were repaired, but were still present in HCT116 cells. Additionally, compared to HCT116 cells, a significantly higher proportion of SW480 cells were ALDH1- and CD44-positive. CONCLUSION: Colon cancers with nuclear β-catenin accumulation demonstrated greater radio-resistance with a higher number of CSCs.
- リンク情報
- ID情報
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- DOI : 10.21873/anticanres.13873
- PubMed ID : 31810923