2019年5月28日
Rapid chemical clearing of white matter in the post-mortem human brain by 1,2-hexanediol delipidation.
Bioorganic & medicinal chemistry letters
- 巻
- 29
- 号
- 15
- 開始ページ
- 1886
- 終了ページ
- 1890
- 記述言語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.bmcl.2019.05.049
Three-dimensional (3D) imaging based on chemical tissue clearing in the post-mortem human brain is a promising approach for stereoscopic understanding of central nervous system diseases. Especially, delipidation of lipid-rich white matter (WM) is a rate-determining step in human brain clearing by hydrophilic reagents. In this study, we described the rapid delipidation of WM by a 1,2-hexanediol (HxD)-based aqueous solution. HxD delipidation enabled rapid clearing of a formalin-fixed human brain specimen including the WM. Although harsh HxD delipidation was applied to the brain tissue, conventional pathological staining patterns and various types of antigenicity were sufficiently preserved. Furthermore, HxD delipidation was compatible with 3D imaging of fluorescently-labeled tissue samples. HxD delipidation could be useful in future 3D neuropathological diagnosis.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.bmcl.2019.05.049
- ISSN : 0960-894X
- ORCIDのPut Code : 80476597
- PubMed ID : 31160178