論文

査読有り
2020年8月3日

ERdj8 governs the size of autophagosomes during the formation process

Journal of Cell Biology
  • Yo-hei Yamamoto
  • ,
  • Ayano Kasai
  • ,
  • Hiroko Omori
  • ,
  • Tomoe Takino
  • ,
  • Munechika Sugihara
  • ,
  • Tetsuo Umemoto
  • ,
  • Maho Hamasaki
  • ,
  • Tomohisa Hatta
  • ,
  • Tohru Natsume
  • ,
  • Richard I. Morimoto
  • ,
  • Ritsuko Arai
  • ,
  • Satoshi Waguri
  • ,
  • Miyuki Sato
  • ,
  • Ken Sato
  • ,
  • Shoshana Bar-Nun
  • ,
  • Tamotsu Yoshimori
  • ,
  • Takeshi Noda
  • ,
  • Kazuhiro Nagata

219
8
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.1083/jcb.201903127
出版者・発行元
Rockefeller University Press

In macroautophagy, membrane structures called autophagosomes engulf substrates and deliver them for lysosomal degradation. Autophagosomes enwrap a variety of targets with diverse sizes, from portions of cytosol to larger organelles. However, the mechanism by which autophagosome size is controlled remains elusive. We characterized a novel ER membrane protein, ERdj8, in mammalian cells. ERdj8 localizes to a meshwork-like ER subdomain along with phosphatidylinositol synthase (PIS) and autophagy-related (Atg) proteins. ERdj8 overexpression extended the size of the autophagosome through its DnaJ and TRX domains. ERdj8 ablation resulted in a defect in engulfing larger targets. C. elegans, in which the ERdj8 orthologue dnj-8 was knocked down, could perform autophagy on smaller mitochondria derived from the paternal lineage but not the somatic mitochondria. Thus, ERdj8 may play a critical role in autophagosome formation by providing the capacity to target substrates of diverse sizes for degradation.

リンク情報
DOI
https://doi.org/10.1083/jcb.201903127
URL
http://rupress.org/jcb/article-pdf/doi/10.1083/jcb.201903127/1044877/jcb_201903127.pdf

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