2021年1月20日
A ClearSee-Based Clearing Protocol for 3D Visualization of Arabidopsis thaliana Embryos
Plants
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- 巻
- 10
- 号
- 2
- 開始ページ
- 190
- 終了ページ
- 190
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.3390/plants10020190
- 出版者・発行元
- MDPI AG
Tissue clearing methods combined with confocal microscopy have been widely used for studying developmental biology. In plants, ClearSee is a reliable clearing method that is applicable to a wide range of tissues and is suitable for gene expression analysis using fluorescent reporters, but its application to the Arabidopsis thaliana embryo, a model system to study morphogenesis and pattern formation, has not been described in the original literature. Here, we describe a ClearSee-based clearing protocol which is suitable for obtaining 3D images of Arabidopsis thaliana embryos. The method consists of embryo dissection, fixation, washing, clearing, and cell wall staining and enables high-quality 3D imaging of embryo morphology and expression of fluorescent reporters with the cellular resolution. Our protocol provides a reliable method that is applicable to the analysis of morphogenesis and gene expression patterns in Arabidopsis thaliana embryos.
- リンク情報
- ID情報
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- DOI : 10.3390/plants10020190
- eISSN : 2223-7747
- PubMed ID : 33498275
- PubMed Central 記事ID : PMC7909245