論文

査読有り 国際誌
2021年1月20日

A ClearSee-Based Clearing Protocol for 3D Visualization of Arabidopsis thaliana Embryos

Plants
  • Ayame Imoto
  • ,
  • Mizuki Yamada
  • ,
  • Takumi Sakamoto
  • ,
  • Airi Okuyama
  • ,
  • Takashi Ishida
  • ,
  • Shinichiro Sawa
  • ,
  • Mitsuhiro Aida

10
2
開始ページ
190
終了ページ
190
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3390/plants10020190
出版者・発行元
MDPI AG

Tissue clearing methods combined with confocal microscopy have been widely used for studying developmental biology. In plants, ClearSee is a reliable clearing method that is applicable to a wide range of tissues and is suitable for gene expression analysis using fluorescent reporters, but its application to the Arabidopsis thaliana embryo, a model system to study morphogenesis and pattern formation, has not been described in the original literature. Here, we describe a ClearSee-based clearing protocol which is suitable for obtaining 3D images of Arabidopsis thaliana embryos. The method consists of embryo dissection, fixation, washing, clearing, and cell wall staining and enables high-quality 3D imaging of embryo morphology and expression of fluorescent reporters with the cellular resolution. Our protocol provides a reliable method that is applicable to the analysis of morphogenesis and gene expression patterns in Arabidopsis thaliana embryos.

リンク情報
DOI
https://doi.org/10.3390/plants10020190
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33498275
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7909245
URL
https://www.mdpi.com/2223-7747/10/2/190/pdf
ID情報
  • DOI : 10.3390/plants10020190
  • eISSN : 2223-7747
  • PubMed ID : 33498275
  • PubMed Central 記事ID : PMC7909245

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