2007年8月
Crystallization and preliminary X-ray diffraction analysis of the cytosolic domain of the Mg2+ transporter MgtE
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
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- 巻
- 63
- 号
- Pt 8
- 開始ページ
- 678
- 終了ページ
- 681
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1107/S1744309107032344
- 出版者・発行元
- BLACKWELL PUBLISHING
The MgtE family of Mg2+ transporters is ubiquitously conserved in all domains of life. The cytosolic domains of the MgtE Mg2+ transporters include a cystathionine-beta-synthase (CBS) domain which is known to play a regulatory function in transporter proteins. The cytosolic domain of MgtE from Thermus thermophilus was overexpressed, purified and crystallized in the presence and absence of Mg2+. The crystals formed in the presence of Mg2+ diffracted X-rays to 2.3 A resolution using synchrotron radiation, belong to space group P6(5)22 with unit-cell parameters a = b = 57.7, c = 317.6 A and are expected to contain one molecule in the asymmetric unit. The crystals formed in the absence of Mg2+ diffracted X-rays to 3.5 A resolution using synchrotron radiation, belong to space group P2(1)2(1)2(1) with unit-cell parameters a = 77.0, b = 100.3, c = 100.3 A and are expected to contain two molecules in the asymmetric unit.
- リンク情報
- ID情報
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- DOI : 10.1107/S1744309107032344
- ISSN : 1744-3091
- PubMed ID : 17671366
- Web of Science ID : WOS:000248441400010