2015年12月1日
The role of toll-like receptors and myeloid differentiation factor 88 in bjerkandera adusta-induced lung inflammation
International Archives of Allergy and Immunology
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- 巻
- 168
- 号
- 2
- 開始ページ
- 96
- 終了ページ
- 106
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1159/000441895
- 出版者・発行元
- KARGER
Background: Recently, a cluster of patients with an intractable allergic fungal cough who were characterized by sensitization to Bjerkandera adusta was reported. In the present study, the role of Toll-like receptors and myeloid differentiation factor 88 (MyD88) in B. adusta-induced lung inflammation was investigated. Methods: Wild-type (WT), TLR2-/- , TLR4-/-, and M yD88-/-BALB/c mice were intratracheally challenged with B. adusta 4 times at 2-week intervals. Lung pathology, bronchoalveolar lavage fluid (BALF) cytological profiles, and inflammatory mediators in BALF were investigated. Bone marrow-derived macrophages (BMDM) from TLR2-/-, T LR4-/-, TLR2/4-/-, T LR7/9-/-, M yD88-/-, and WT C57BL/6J mice were stimulated with B. adusta for 12 h, and inflammatory mediators in the culture medium were measured. Results: B. adusta caused lung inflammation along with Th2 cytokine [interleukin (IL)-5 and IL-13] and eosinophil-related chemokine [eotaxin and monocyte chemotactic protein (MCP-3)] production, an increase in eosinophils in BALF, and eosinophil infiltration in the airways in WT and TLR4-/- mice. However, Th2 and eosinophil-related responses in TLR2-/- and MyD88-/- mice were low or undetectable. The induction of neutrophils and IL-6, IL-12, IL-17A, and MCP-1 in the BALF of MyD88-/- mice was attenuated compared to that in WT mice. The induction of IL-6, TNF-?, MCP-1, and macrophage inflammatory protein-1? was reduced or undetectable in B. adusta-stimulated BMDM from TLR7/9-/-and MyD88-/- mice compared to WT mice. Conclusions: These results suggest that TLR2 and the adapter protein MyD88 may play an important role in the induction of eosinophils by B. adusta. However, TLR7/9-MyD88 might be important in the induction of neutrophils and the relevant inflammatory mediators, especially IL-17A.
- リンク情報
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- DOI
- https://doi.org/10.1159/000441895
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/26641462
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000368446900004&DestApp=WOS_CPL
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84949556448&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84949556448&origin=inward
- URL
- http://europepmc.org/abstract/med/26641462
- ID情報
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- DOI : 10.1159/000441895
- ISSN : 1018-2438
- eISSN : 1423-0097
- ORCIDのPut Code : 64433482
- PubMed ID : 26641462
- SCOPUS ID : 84949556448
- Web of Science ID : WOS:000368446900004