We consider the Standard Model extended by right-handed neutrinos to explain massive neutrinos through the seesaw mechanism. The new fermion can be observed when it has a sufficiently small mass and large mixings to left-handed neutrinos. If such a particle is the lightest right-handed neutrino, its contribution to the mass matrix of active neutrinos needs to be canceled by that of a heavier one. Yukawa couplings of the heavier one are then larger than those of the lightest one. We show that the perturbativity condition gives a severe upper bound on the mixing of the lightest right-handed neutrino, depending on the masses of heavier ones. Models of high energy phenomena, such as leptogenesis, can be constrained by low energy experiments. (C) 2015 The Authors. Published by Elsevier B.V.