2013年
Soaking RNAi in Bombyx mori BmN4-SID1 cells arrests cell cycle progression
Journal of Insect Science
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- 巻
- 13
- 号
- 開始ページ
- 155
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1673/031.013.15501
- 出版者・発行元
- Library of the University of Arizona
RNA interference (RNAi) is an evolutionarily conserved mechanism for sequence-specific gene silencing. Previously, the BmN4-SID1 cell expressing Caenorhabditis ele gans SID-1 was established, in which soaking RNAi could induce effective gene silencing. To establish its utility, 6 cell cycle progression related cDNAs, CDK1, MYC, MYB, RNRS, CDT1, and GEMININ, were isolated from the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae), and their expressions were further silenced by soaking RNAi in the BmN4-SID1 cells. The cell cycle progression analysis using flow cytometer demonstrated that the small amount of double stranded RNA was enough to arrest cell cycle progression at the specific cell phases. These data suggest that RNAi in the BmN4-SID1 cells can be used as a powerful tool for loss-of-function analysis of B. mori genes.© 2013 Journal of Insect Science.
- リンク情報
- ID情報
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- DOI : 10.1673/031.013.15501
- ISSN : 1536-2442
- PubMed ID : 24773378
- SCOPUS ID : 84907311308
- Web of Science ID : WOS:000329281400001