論文

国際誌
2021年4月

In vivo regeneration of rat laryngeal cartilage with mesenchymal stem cells derived from human induced pluripotent stem cells via neural crest cells.

Stem cell research
  • Masayoshi Yoshimatsu
  • ,
  • Hiroe Ohnishi
  • ,
  • Chengzhu Zhao
  • ,
  • Yasuyuki Hayashi
  • ,
  • Fumihiko Kuwata
  • ,
  • Shinji Kaba
  • ,
  • Hideaki Okuyama
  • ,
  • Yoshitaka Kawai
  • ,
  • Nao Hiwatashi
  • ,
  • Yo Kishimoto
  • ,
  • Tatsunori Sakamoto
  • ,
  • Makoto Ikeya
  • ,
  • Koichi Omori

52
開始ページ
102233
終了ページ
102233
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.scr.2021.102233

The laryngotracheal cartilage is a cardinal framework for the maintenance of the airway for breathing, which occasionally requires reconstruction. Because hyaline cartilage has a poor intrinsic regenerative ability, various regenerative approaches have been attempted to regenerate laryngotracheal cartilage. The use of autologous mesenchymal stem cells (MSCs) for cartilage regeneration has been widely investigated. However, long-term culture may limit proliferative capacity. Human-induced pluripotent stem cell-derived MSCs (iMSCs) can circumvent this problem due to their unlimited proliferative capacity. This study aimed to investigate the efficacy of iMSCs in the regeneration of thyroid cartilage in immunodeficient rats. Herein, we induced iMSCs through neural crest cell intermediates. For the relevance to prospective future clinical application, induction was conducted under xeno-free/serum-free conditions. Then, clumps fabricated from an iMSC/extracellular matrix complex (C-iMSC) were transplanted into thyroid cartilage defects in immunodeficient rats. Histological examinations revealed cartilage-like regenerated tissue and human nuclear antigen (HNA)-positive surviving transplanted cells in the regenerated lesion. HNA-positive cells co-expressed SOX9, and type II collagen was identified around HNA-positive cells. These results indicated that the transplanted C-iMSCs promoted thyroid cartilage regeneration and some of the iMSCs differentiated into chondrogenic lineage cells. Induced MSCs may be a promising candidate cell therapy for human laryngotracheal reconstruction.

リンク情報
DOI
https://doi.org/10.1016/j.scr.2021.102233
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33607469
ID情報
  • DOI : 10.1016/j.scr.2021.102233
  • PubMed ID : 33607469

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