論文

査読有り 国際誌
2019年10月30日

Roles of monocarboxylate transporter subtypes in promotion and suppression of osteoclast differentiation and survival on bone.

Scientific Reports
  • Hiroko Imai
  • ,
  • Kentaro Yoshimura
  • ,
  • Yoichi Miyamoto
  • ,
  • Kiyohito Sasa
  • ,
  • Marika Sugano
  • ,
  • Masahiro Chatani
  • ,
  • Masamichi Takami
  • ,
  • Matsuo Yamamoto
  • ,
  • Ryutaro Kamijo

9
1
開始ページ
15608
終了ページ
15608
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-019-52128-2

Monocarboxylate transporters (MCTs) provide transmembrane transport of monocarboxylates such as lactate and pyruvate. The present results showed that α-cyano-4-hydroxycinnamic acid (CHC), an inhibitor of MCTs, promoted osteoclast differentiation from macrophages at lower concentrations (0.1-0.3 mM) and suppressed that at a higher concentration (1.0 mM). On the other hand, CHC reduced the number of mature osteoclasts on the surface of dentin in a concentration-dependent manner. Additionally, macrophages and osteoclasts were found to express the Mct1, Mct2, and Mct4 genes, with Mct1 and Mct4 expression higher in macrophages, and that of Mct2 higher in osteoclasts. Although Mct1 gene knockdown in macrophages enhanced osteoclast formation induced by RANKL, Mct2 gene knockdown suppressed that. Finally, Mct2 gene silencing in mature osteoclasts decreased their number and, thereby, bone resorption. These results suggest that MCT1 is a negative regulator and MCT2 a positive regulator of osteoclast differentiation, while MCT2 is required for bone resorption by osteoclasts.

リンク情報
DOI
https://doi.org/10.1038/s41598-019-52128-2
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31666601
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6821745

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