2020年
Lipoprotein profile and lipid metabolism of PXB-cells®, human primary hepatocytes from liver-humanized mice: proposal of novel in vitro system for screening anti-lipidemic drugs
Biomedical Research (Japan)
- 巻
- 41
- 号
- 1
- 開始ページ
- 33
- 終了ページ
- 42
- 記述言語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.2220/biomedres.41.33
© 2020, Biomedical Research Foundation. All rights reserved. We investigated lipid metabolism in PXB-cells, which are human primary hepatocytes isolated from liver-humanized mice, and HepG2 and HuH-7 human hepatoma cell lines. Lipoprotein levels were higher in PXB-cells than in the 2 other cell lines, and PXB-cells mainly released triglycerides and cholesterol as very low density lipoprotein (VLDL), similar to actual liver tissue, whereas the major lipoprotein released from the 2 hepatoma cell lines was LDL. RT-PCR analysis demonstrated that the gene expression levels of apolipoprotein B100 (ApoB100), the apolipoprotein of VLDL/LDL, were similar in PXB-cells and HepG2 cells, while the overexpression of ApoC2, ApoC3, and ApoE, which are components of VLDL, but not LDL, was observed in PXB-cells. A protein immunoassay revealed that ApoB100 levels secreted from PXB-cells and HuH-7 cells were similar; however, ApoC3 levels were higher in PXB-cells than in the two other cell lines. We also examined the anti-lipidemic activities of fenofibrate using this assay system. Fenofibrate suppressed lipoprotein production from PXB-cells in a dose-dependent manner mainly by activating the β-oxidation pathway. These results suggest that PXB-cells produce high levels of lipoproteins and are suitable for screening anti-lipidemic agents.
- リンク情報
-
- DOI
- https://doi.org/10.2220/biomedres.41.33
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/32092738
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85079832834&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85079832834&origin=inward
- ID情報
-
- DOI : 10.2220/biomedres.41.33
- ISSN : 0388-6107
- eISSN : 1880-313X
- PubMed ID : 32092738
- SCOPUS ID : 85079832834