2017年8月
Crystal structure of octocoral lectin SLL-2 complexed with Forssman antigen tetrasaccharide
GLYCOBIOLOGY
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- 巻
- 27
- 号
- 8
- 開始ページ
- 696
- 終了ページ
- 700
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/glycob/cwx043
- 出版者・発行元
- OXFORD UNIV PRESS INC
A symbiosis-related lectin, SLL-2, from the octocoral Sinularia lochmodes, distributes densely on the cell surface of microalgae, Symbiodinium sp., an endosymbiotic dinoflagellate of the coral, and is also shown to be a chemical cue that transforms dinoflagellates into a nonmotile (coccoid) symbiotic state. SLL-2 binds to the sugar chain of the molecule similar to Forssman antigen pentasaccharide (GalNAc alpha 1-3GalNAc beta 1-3Gala1-4Gal alpha 1-4Glc) on the surface of microalgae with high affinity. Here we report the crystal structure of the complex between SLL-2 and Forssman antigen tetrasaccharide (GalNAc alpha 1-3GalNAc beta 1-3Gala1-4Gal beta) at 3.4 angstrom resolution. In an asymmetric unit of the crystal, there are two hexameric molecules with totally 12 sugar recognition sites. At 9 in 12 sites, the first and second saccharides of the Forssman antigen tetrasaccharide bind directly to galactopyranoside binding site of SLL-2, whereas the third and fourth saccharides have no interaction with the SLL-2 hexameric molecule that binds the first saccharide. The sugar chain bends at alpha-1,4-glycosidic linkage between the third and fourth saccharides toward the position that we defined as a pyranoside binding site in the crystal structure of the complex between SLL-2 and GalNAc. The structure allowed us to suggest a possible binding mode of the Forssman antigen pentasaccharide to SLL-2. These observations support our hypothesis that the binding of SLL-2 to the cell surface sugars of zooxanthella in a unique manner might trigger some physiological changes of the cell to adapt symbiosis with the host coral.
- リンク情報
-
- DOI
- https://doi.org/10.1093/glycob/cwx043
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000407146700002&DestApp=WOS_CPL
- URL
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85028361757&origin=inward
- URL
- http://orcid.org/0000-0003-1919-6309
- ID情報
-
- DOI : 10.1093/glycob/cwx043
- ISSN : 0959-6658
- eISSN : 1460-2423
- ORCIDのPut Code : 46070460
- SCOPUS ID : 85028361757
- Web of Science ID : WOS:000407146700002