In the present work oil-in-gel (o/g) emulsions comprising 0.4-1% w/w K-carrageenan in the absence and presence of Ca2+ (20 mM) were investigated for their ability to hamper the oxidative degradation under isothermal (5, 20 and 37 degrees C) ambient air storage conditions and promote the in vitro bioaccessibility of beta-carotene. The mechanical and structure conformational aspects of the o/g emulsions throughout in vitro digestion were measured by means of oscillatory rheology and optical microscopy. Although the addition of kappa-carrageenan reduced by more than 50% (from 0.85 to 0.40 day(-1)) the p-carotene oxidative degradation rates, only minor differences were detected in terms of gelation method (ca. 0.42 and 0.39 day(-1) for ionotropic and coldset systems) and biopolymer concentration (0.38-0.42 day(-1)). The bioaccessibility of beta-carotene was significantly higher in coldset o/g emulsions (ca. 43%) compared to the ionically mediated ones (ca. 36%). With the exception of 0.4% K-carrageenan containing o/g emulsions (55.4 and 49.7% for control and 0.4% K-carrageenan respectively), the p-carotene bioaccessibility was significantly reduced with the increase in K-carrageenan concentration (ranging from 34 to 38.9%). (C) 2017 Elsevier Ltd. All rights reserved.