2013年1月
Probing the function of neuronal populations: Combining micromirror-based optogenetic photostimulation with voltage-sensitive dye imaging
NEUROSCIENCE RESEARCH
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- 巻
- 75
- 号
- 1
- 開始ページ
- 76
- 終了ページ
- 81
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.neures.2012.11.006
- 出版者・発行元
- ELSEVIER IRELAND LTD
Recent advances in our understanding of brain function have come from using light to either control or image neuronal activity. Here we describe an approach that combines both techniques: a micromirror array is used to photostimulate populations of presynaptic neurons expressing channelrhodopsin-2, while a red-shifted voltage-sensitive dye allows optical detection of resulting postsynaptic activity. Such technology allowed us to control the activity of cerebellar interneurons while simultaneously recording inhibitory responses in multiple Purkinje neurons, their postsynaptic targets. This approach should substantially accelerate our understanding of information processing by populations of neurons within brain circuits. (C) 2013 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.neures.2012.11.006
- ISSN : 0168-0102
- eISSN : 1872-8111
- PubMed ID : 23254260
- Web of Science ID : WOS:000316435700013