論文

査読有り 国際誌
2020年5月6日

Biomimetic aorta-gonad-Mesonephros-on-a-Chip to study human developmental hematopoiesis.

Biomedical microdevices
  • Ryohichi Sugimura
  • ,
  • Ryo Ohta
  • ,
  • Chihiro Mori
  • ,
  • Alina Li
  • ,
  • Takafumi Mano
  • ,
  • Emi Sano
  • ,
  • Kaori Kosugi
  • ,
  • Tatsutoshi Nakahata
  • ,
  • Akira Niwa
  • ,
  • Megumu K Saito
  • ,
  • Yu-Suke Torisawa

22
2
開始ページ
34
終了ページ
34
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s10544-020-00488-2

A fundamental limitation in the derivation of hematopoietic stem and progenitor cells is the imprecise understanding of human developmental hematopoiesis. Herein we established a multilayer microfluidic Aorta-Gonad-Mesonephros (AGM)-on-a-chip to emulate developmental hematopoiesis from pluripotent stem cells. The device consists of two layers of microchannels separated by a semipermeable membrane, which allows the co-culture of human hemogenic endothelial (HE) cells and stromal cells in a physiological relevant spatial arrangement to replicate the structure of the AGM. HE cells derived from human induced pluripotent stem cells (hiPSCs) were cultured on a layer of mesenchymal stromal cells in the top channel while vascular endothelial cells were co-cultured on the bottom side of the membrane within the microfluidic device. We show that this AGM-on-a-chip efficiently derives endothelial-to-hematopoietic transition (EHT) from hiPSCs compared with regular suspension culture. The presence of mesenchymal stroma and endothelial cells renders functional HPCs in vitro. We propose that the AGM-on-a-chip could serve as a platform to dissect the cellular and molecular mechanisms of human developmental hematopoiesis.

リンク情報
DOI
https://doi.org/10.1007/s10544-020-00488-2
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32377802

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