2005年10月
An in vitro RNA editing system from cauliflower mitochondria: Editing site recognition parameters can vary in different plant species
RNA-A PUBLICATION OF THE RNA SOCIETY
- ,
- ,
- ,
- 巻
- 11
- 号
- 10
- 開始ページ
- 1563
- 終了ページ
- 1570
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1261/rna.2740905
- 出版者・発行元
- COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
Most of the 400 RNA editing sites in flowering plant mitochondria are found in mRNAs. Consequently, the sequence vicinities of homologous sites are highly conserved between different species and are presumably recognized by likewise conserved transfactors. To investigate the evolutionary adaptation to sequence variation, we have now analyzed the recognition elements of an editing site with divergent upstream sequences in the two species pea and cauliflower. This variation is tolerated at the site selected, because the upstream cis-elements reach into the 5'-UTR of the mRNA. To compare cis-recognition features in pea and cauliflower mitochondria, we developed a new in vitro RNA editing system for cauliflower. in vitro editing assays with deleted and mutated template RNAs show that the major recognition elements for both species are located within the conserved sequence. In cauliflower, however, the essential upstream nucleotides extend further upstream than they do in pea. In-depth analysis of single-nucleotide mutations reveals critical spacing of the editing site and the specific recognition elements, and shows that the +1 nucleotide identity is important in cauliflower, but not in pea.
- リンク情報
- ID情報
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- DOI : 10.1261/rna.2740905
- ISSN : 1355-8382
- PubMed ID : 16131591
- Web of Science ID : WOS:000232330200011