2015年7月
Suppression of intestinal carcinogenesis in Apc-mutant mice by limonin
JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION
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- 巻
- 57
- 号
- 1
- 開始ページ
- 39
- 終了ページ
- 43
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.3164/jcbn.15-28
- 出版者・発行元
- JOURNAL CLINICAL BIOCHEMISTRY & NUTRITION
Limonoids in citrus fruits are known to possess multiple biological functions, such as anti-proliferative functions in human cancer cell lines. Therefore, we aimed to investigate the suppressive effect of limonin on intestinal polyp development in Apc-mutant Min mice. Five-week-old female Min mice were fed a basal diet or a diet containing 250 or 500 ppm limonin for 8 weeks. The total number of polyps in mice treated with 500 ppm limonin decreased to 74% of the untreated control value. Neoplastic cell proliferation in the polyp parts was assessed by counting PCNA positive cells, and a tendency of reduction was obtained by limonin treatment. Moreover, expression levels of c-Myc and MCP-1 mRNA in the polyp part were reduced by administration of limonin. We finally confirmed the effects of limonin on beta-catenin signaling, and found limonin significantly inhibited T-cell factor/lymphocyte enhancer factor-dependent transcriptional activity in a dose-dependent manner in the Caco-2 human colon cancer cell line. Our results suggest that limonin might be a candidate chemopreventive agent against intestinal carcinogenesis.
- リンク情報
- ID情報
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- DOI : 10.3164/jcbn.15-28
- ISSN : 0912-0009
- eISSN : 1880-5086
- PubMed ID : 26236099
- Web of Science ID : WOS:000358457800006