Nov, 2019
Novel neuroprotective hydroquinones with a vinyl alkyne from the fungus, Pestalotiopsis microspora.
The Journal of antibiotics
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- Volume
- 72
- Number
- 11
- First page
- 793
- Last page
- 799
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1038/s41429-019-0213-9
New hydroquinone derivatives bearing a vinyl alkyne, pestalotioquinols A and B, were isolated from a fungal culture broth of Pestalotiopsis microspora. The structures of these novel compounds were determined by interpretation of spectroscopic data (1D/2D NMR, MS, and IR), and the absolute configuration of the stereogenic center of pestalotioquinol A was assigned using the modified Mosher's method. Nerve growth factor-differentiated neuronal PC12 cells were pretreated with pestalotioquinols A and B and removed from the medium, and then treated with a generator of peroxynitrite (ONOO-), a reactive nitrogen species, to induce cell death. The cytotoxicity of the treated cells was assessed by measuring lactate dehydrogenase leakage. As a result, 1-3 μM pretreatment of pestalotioquinols A and B rescued neuronal PC12 cells from peroxynitrite-induced cytotoxicity and the protective activity was sustained after removing each compound from the medium. These results demonstrate that pestalotioquinol derivatives are a new class of hydroquinones possessing a vinyl alkyne and exhibiting relatively high neuroprotective effects.
- Link information
- ID information
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- DOI : 10.1038/s41429-019-0213-9
- ISSN : 0021-8820
- eISSN : 1881-1469
- Pubmed ID : 31341274
- SCOPUS ID : 85069631973