2007
Efficient elimination of multidrug-resistant Staphylococcus aureus by cloned lysin derived from bacteriophage φMR11
Journal of Infectious Diseases
- Volume
- 196
- Number
- 8
- First page
- 1237
- Last page
- 1247
- Language
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1086/521305
- Publisher
- The University of Chicago Press
We report the successful purification of a cloned lysin encoded by the novel Staphylococcus aureus bacteriophage φMR11. The lysin, designated MV-L, rapidly and completely lysed cells of a number of S. aureus strains tested, including methicillin-resistant S. aureus (MRSA) and vancomycin-resistant S. aureus and a subset of vancomycin-intermediate S. aureus (VISA) in growing conditions. MV-L-mediated killing is specific to S. aureus and not to other species, except for S. simulans. MV-L exerted its staphylocidal effect synergistically with glycopeptide antibiotics against VISA. MV-L efficiently eliminated MRSA that had been artificially inoculated into the nares of mice. The intraperitoneal administration of MV-L also protected mice against MRSA septic death, without any harmful effects. Although MV-L evoked detectable levels of a humoral response in mice, the antibodies did not abolish the bacteriolytic activity. These results indicate that MV-L might be useful as a powerful therapeutic agent against multidrug-resistant S. aureus infections. © 2007 by the Infectious Diseases Society of America. All rights reserved.
- Link information
-
- DOI
- https://doi.org/10.1086/521305
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/17955443
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000249595700017&DestApp=WOS_CPL
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=35348839572&origin=inward Open access
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=35348839572&origin=inward
- ID information
-
- DOI : 10.1086/521305
- ISSN : 0022-1899
- ORCID - Put Code : 49158209
- Pubmed ID : 17955443
- SCOPUS ID : 35348839572
- Web of Science ID : WOS:000249595700017