2004年6月
Structural basis of long-term potentiation in single dendritic spines
NATURE
- ,
- ,
- ,
- 巻
- 429
- 号
- 6993
- 開始ページ
- 761
- 終了ページ
- 766
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/nature02617
- 出版者・発行元
- NATURE PUBLISHING GROUP
Dendritic spines of pyramidal neurons in the cerebral cortex undergo activity-dependent structural remodelling(1-5) that has been proposed to be a cellular basis of learning and memory(6). How structural remodelling supports synaptic plasticity(4),5, such as long-term potentiation(7), and whether such plasticity is input-specific at the level of the individual spine has remained unknown. We investigated the structural basis of long-term potentiation using two-photon photolysis of caged glutamate at single spines of hippocampal CA1 pyramidal neurons(8). Here we show that repetitive quantum-like photorelease (uncaging) of glutamate induces a rapid and selective enlargement of stimulated spines that is transient in large mushroom spines but persistent in small spines. Spine enlargement is associated with an increase in AMPA-receptor-mediated currents at the stimulated synapse and is dependent on NMDA receptors, calmodulin and actin polymerization. Long-lasting spine enlargement also requires Ca2+/calmodulin-dependent protein kinase II. Our results thus indicate that spines individually follow Hebb's postulate for learning. They further suggest that small spines are preferential sites for long-term potentiation induction, whereas large spines might represent physical traces of long-term memory.
- リンク情報
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- DOI
- https://doi.org/10.1038/nature02617
- CiNii Articles
- http://ci.nii.ac.jp/naid/80016655732
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/15190253
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000222059900039&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-3042554012&partnerID=MN8TOARS
- URL
- http://orcid.org/0000-0003-2327-9027
- ID情報
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- DOI : 10.1038/nature02617
- ISSN : 0028-0836
- eISSN : 1476-4687
- CiNii Articles ID : 80016655732
- ORCIDのPut Code : 19717507
- PubMed ID : 15190253
- SCOPUS ID : 3042554012
- Web of Science ID : WOS:000222059900039