2016年10月
A New In Vitro Co-Culture Model Using Magnetic Force-Based Nanotechnology
JOURNAL OF CELLULAR PHYSIOLOGY
- 巻
- 231
- 号
- 10
- 開始ページ
- 2249
- 終了ページ
- 2256
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/jcp.25342
- 出版者・発行元
- WILEY-BLACKWELL
Skeletal myoblast (SkMB) transplantation has been conducted as a therapeutic strategy for severe heart failure. However, arrhythmogenicity following transplantation remains unsolved. We developed an in vitro model of myoblast transplantation with patterned or randomly-mixed co-culture of SkMBs and cardiomyocytes enabling subsequent electrophysiological, and arrhythmogenic evaluation. SkMBs were magnetically labeled with magnetite nanoparticles and co-cultured with neonatal rat ventricular myocytes (NRVMs) on multi-electrode arrays. SkMBs were patterned by a magnet beneath the arrays. Excitation synchronicity was evaluated by Ca2+ imaging using a gene-encoded Ca2+ indicator, G-CaMP2. In the monoculture of NRVMs (control), conduction was well-organized. In the randomly-mixed co-culture of NRVMs and SkMBs (random group), there was inhomogeneous conduction from multiple origins. In the patterned co-culture where an en bloc SKMB-layer was inserted into the NRVM-layer, excitation homogenously propagated although conduction was distorted by the SkMB-area. The 4-mm distance conduction time (CT) in the random group was significantly longer (197 +/- 126ms) than in control (17 +/- 3ms). In the patterned group, CT through NRVM-area did not change (25 +/- 3ms), although CT through the SkMB-area was significantly longer (132 +/- 77ms). The intervals between spontaneous excitation varied beat-to-beat in the random group, while regular beating was recorded in the control and patterned groups. Synchronized Ca2+ transients of NRVMs were observed in the patterned group, whereas those in the random group were asynchronous. Patterned alignment of SkMBs is feasible with magnetic nanoparticles. Using the novel in vitro model mimicking cell transplantation, it may become possible to predict arrhythmogenicity due to heterogenous cell transplantation. J. Cell. Physiol. 231: 2249-2256, 2016. (c) 2016 Wiley Periodicals, Inc.
- リンク情報
- ID情報
-
- DOI : 10.1002/jcp.25342
- ISSN : 0021-9541
- eISSN : 1097-4652
- PubMed ID : 26873862
- Web of Science ID : WOS:000379159500018