2011年7月26日
Single chain variable fragment antibodies against shiga toxins isolated from a human antibody phage display library
Vaccine
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- 巻
- 29
- 号
- 33
- 開始ページ
- 5340
- 終了ページ
- 5346
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.vaccine.2011.05.093
- 出版者・発行元
- ELSEVIER SCI LTD
Shiga toxins (Stxs) are involved in the pathogenesis of hemolytic-uremic syndrome and other severe systemic complications following enterohemorrhagic Escherichia coli infection in humans. Passive immunotherapies using monoclonal antibodies have been shown to be effective for neutralizing the toxic effects of Stxs. However, animal-derived monoclonal antibodies are sometimes immunogenic and their production is both laborious and expensive. We here report the isolation of single-chain variable fragment antibodies against Stxs by screening a phage display library constructed from a naïve human repertoire. An antibody among the selected clones designated B22 bound to the binding subunits of both Stx-1 and Stx-2, and strongly neutralized the cytotoxicity of Stx-1. This is the first example of a monovalent antibody showing Stx-neutralizing activity. The B22 antibody is also completely naturally occurring in human, which reduces the possibility of adverse immunological effects, and can be easily produced using bacterial protein synthesis systems. © 2011 Elsevier Ltd.
- リンク情報
-
- DOI
- https://doi.org/10.1016/j.vaccine.2011.05.093
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/21664401
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000293765300002&DestApp=WOS_CPL
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79960380767&origin=inward
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=79960380767&origin=inward
- ID情報
-
- DOI : 10.1016/j.vaccine.2011.05.093
- ISSN : 0264-410X
- eISSN : 1873-2518
- PubMed ID : 21664401
- SCOPUS ID : 79960380767
- Web of Science ID : WOS:000293765300002