Motomitsu Kitaoka

Profile Information

Affiliation: Faculty of Agriculture, Niigata University

Degree: Ph. D.

ORCID ID: https://orcid.org/0000-0002-0083-1838
J-GLOBAL ID: 201201040378524542
researchmap Member ID: B000220610

糖加リン酸分解酵素(ホスホリラーゼ）などの糖とリン酸に関わる酵素を中心にして、種々の糖質関連酵素を利用した実用的なオリゴ糖製造方法の開発を行っています。また、ビフィズス菌のヒトミルクオリゴ糖代謝に関わる酵素群の同定及びその利用に関する研究を行っています。

Research Interests

Research Areas

Life sciences / Applied biochemistry / Enzymology

Research History

Apr, 2019 - Present

Professor, Faculty of Agriculture, Niigata University
Apr, 2018 - Mar, 2019

Director, Division of Analytical Science, Food Research Institute, National Agriculture and Food Research Organization
Apr, 2015 - Mar, 2018

Head, Enzyme Research Unit, Division of Food Biotechnology, Food Research Institute, National Agriculture and Food Research Organization
Apr, 2006 - Mar, 2015

Leader, Enzyme Laboratory, Food Biotechnology Division, National Food Research Institute, NARO
Apr, 2004 - Mar, 2006

Leader, Enzyme Laboratory, Division of Biotechnology, National Food Research Institute
Apr, 2001 - Mar, 2004

Chief Researcher, Department of Biotechnology, National Food Research Institute
Oct, 1999 - Mar, 2001

Chief Researcher, Department of Biotechnology, National Food Research Institute
Apr, 1998 - Sep, 1999

Postdoctral Fellow, (Natioal Food Research Institute), Bio-oriented Technology Research Advancement Institution
May, 1995 - Feb, 1998

Postdoctral Fellow, Department of Biochemistry and Biophysics, Iowa State University

Awards

Sep, 2017

Society Award, Advanced utilization of carbohydrate-processing enzymes, The Japanese Society of Applied Glycoscience

Motomitsu Kitaoka
Mar, 2011

Most Cited Paper Award, doi: 10.1271/bbb.80697, The Japan Society for Bioscience, Biotechnology, and Agrochemistry

Motomitsu Kitaoka
Apr, 2009

Technology Award, Production of lacto-N-biose I, the bifidus factor in human milk oligosaccharides, The Iijima Foundation for Food Science and Technology,

Motomitsu Kitaoka
Mar, 2009

Most Cited Paper Award, doi: 10.1271/bbb.70320, The Japan Society for Bioscience, Biotechnology, and Agrochemistry

Motomitsu Kitaoka
Sep, 2002

Society Award for the Encouragement of Young Scientists, Studies on Carbohydrate-processing Phosphorolytic Enzymes, The Japanese Society of Applied Glycoscience

Motomitsu Kitaoka

Papers

214

Extracellular oil production by Rhodotorula paludigena BS15 for biorefinery without complex downstream processes

Junichi Mano, Hirotoshi Sushida, Tsuyoshi Tanaka, Ken Naito, Hiroshi Ono, Masakazu Ike, Ken Tokuyasu, Motomitsu Kitaoka

Applied Microbiology and Biotechnology, 107(22) 6799-6809, Sep 19, 2023
Encapsulation of Lacto‐N‐biose based on ovalbumin and carboxymethyl cellulose microparticles: fabrication, characterisation and thermal stability

Xin Huang, Yue Sun, Suhong Li, Motomitsu Kitaoka, Tuoping Li

International Journal of Food Science & Technology, 58(5) 2590-2598, May, 2023
Substrate recognition mode of a glycoside hydrolase family 42 β-galactosidase from Bifidobacterium longum subspecies infantis (BiBga42A) revealed by crystallographic and mutational analyses

Aina Gotoh, Masafumi Hidaka, Haruko Sakurama, Mamoru Nishimoto, Motomitsu Kitaoka, Mikiyasu Sakanaka, Shinya Fushinobu, Takane Katayama

Microbiome Research Reports, 2 20, May, 2023 Peer-reviewed
Automatic Calculation of the Kinetic Parameters of Enzymatic Reactions with Their Standard Errors Using Microsoft Excel.

Motomitsu Kitaoka

Journal of applied glycoscience, 70(1) 33-37, 2023
Discovery of solabiose phosphorylase and its application for enzymatic synthesis of solabiose from sucrose and lactose

Wataru Saburi, Takanori Nihira, Hiroyuki Nakai, Motomitsu Kitaoka, Haruhide Mori

Scientific Reports, 12(1), Dec, 2022
Priority effects shape the structure of infant-type Bifidobacterium communities on human milk oligosaccharides.

Miriam N Ojima, Lin Jiang, Aleksandr A Arzamasov, Keisuke Yoshida, Toshitaka Odamaki, Jinzhong Xiao, Aruto Nakajima, Motomitsu Kitaoka, Junko Hirose, Tadasu Urashima …

The ISME journal, 16(9) 2265-2279, Jun 29, 2022
Isomerization of 6-O-substituted glucose and fructose under neutral pH conditions and subsequent β-elimination reactions

Kazuhiro Chiku, Ayaka Ohfuji, Nozomi Ohtake, Mitsuru Yoshida, Hiroshi Ono, Motomitsu Kitaoka

Carbohydrate Research, 519 108626-108626, Jun, 2022
Production of lacto-<i>N</i>-biose I using crude extracts of bifidobacterial cells

Shuntaro Machida, Katsuichi Saito, Mamoru Nishimoto, Motomitsu Kitaoka

Journal of Applied Glycoscience, 69(2) 15-21, Feb 25, 2022
Diversification of a Fucosyllactose Transporter within the Genus Bifidobacterium.

Miriam N Ojima, Yuya Asao, Aruto Nakajima, Toshihiko Katoh, Motomitsu Kitaoka, Aina Gotoh, Junko Hirose, Tadasu Urashima, Satoru Fukiya, Atsushi Yokota …

Applied and environmental microbiology, 88(2) e0143721, Jan 25, 2022
Identification of difructose dianhydride I synthase/hydrolase from an oral bacterium establishes a novel glycoside hydrolase family

Toma Kashima, Kouki Okumura, Akihiro Ishiwata, Machika Kaieda, Tohru Terada, Takatoshi Arakawa, Chihaya Yamada, Kentaro Shimizu, Katsunori Tanaka, Motomitsu Kitaoka …

Journal of Biological Chemistry, 297(5) 101324-101324, Nov, 2021
Three-dimensional alignment of cellulose II microcrystals under a strong magnetic field

Masahisa Wada, Sayuri Wakiya, Kayoko Kobayashi, Satoshi Kimura, Motomitsu Kitaoka, Ryosuke Kusumi, Fumiko Kimura, Tsunehisa Kimura

Cellulose, 28(11) 6757-6765, Jul, 2021
Generation of 3-Deoxypentulose by the Isomerization and β-Elimination of 4-O-Substituted Glucose and Fructose

Kazuhiro Chiku, Mitsuru Yoshida, Hiroshi Ono, Motomitsu Kitaoka

Carbohydrate Research, 108402-108402, Jul, 2021
Next-generation prebiotic promotes selective growth of bifidobacteria, suppressing Clostridioides difficile

Rika Hirano, Mikiyasu Sakanaka, Kazuto Yoshimi, Naohisa Sugimoto, Syogo Eguchi, Yuko Yamauchi, Misaki Nara, Shingo Maeda, Yuta Ami, Aina Gotoh …

Gut Microbes, 13(1), Jan 1, 2021
Conversion of levoglucosan into glucose by the coordination of four enzymes through oxidation, elimination, hydration, and reduction.

Yuya Kuritani, Kohei Sato, Hideo Dohra, Seiichiro Umemura, Motomitsu Kitaoka, Shinya Fushinobu, Nobuyuki Yoshida

Scientific reports, 10(1) 20066-20066, Nov 18, 2020
Alkoxycarbonyl elimination of 3-O-substituted glucose and fructose by heat treatment under neutral pH

Kazuhiro Chiku, Riku Tsukasaki, Yu Teshima, Mitsuru Yoshida, Hiroki Aramasa, Takanori Nihira, Hiroyuki Nakai, Hiroshi Ono, Motomitsu Kitaoka

Carbohydrate Research, 108129-108129, Aug, 2020 Peer-reviewed
Effect of C-6 Methylol Groups on Substrate Recognition of Glucose/Xylose Mixed Oligosaccharides by Cellobiose Dehydrogenase from the Basidiomycete Phanerochaete chrysosporium

Kiyohiko Igarashi, Satoshi Kaneko, Motomitsu Kitaoka, Masahiro Samejima

Journal of Applied Glycoscience, 67(2) 51-57, May 20, 2020
Surface structural analysis of selectively ¹³C-labeled cellulose II by solid-state NMR spectroscopy

Yusuke Kita, Ryosuke Kusumi, Tsunehisa Kimura, Motomitsu Kitaoka, Yusuke Nishiyama, Masahisa Wada

Cellulose, 27(4) 1899-1907, Mar 1, 2020 Peer-reviewed
Varied Pathways of Infant Gut-Associated Bifidobacterium to Assimilate Human Milk Oligosaccharides: Prevalence of the Gene Set and Its Correlation with Bifidobacteria-Rich Microbiota Formation.

Sakanaka M, Gotoh A, Yoshida K, Odamaki T, Koguchi H, Xiao JZ, Kitaoka M, Katayama T

Nutrients, 12(1) 71-71, Dec, 2019 Peer-reviewed
Evolutionary adaptation in fucosyllactose uptake systems supports bifidobacteria-infant symbiosis.

Mikiyasu Sakanaka, Morten Ejby Hansen, Aina Gotoh, Toshihiko Katoh, Keisuke Yoshida, Toshitaka Odamaki, Hiroyuki Yachi, Yuta Sugiyama, Shin Kurihara, Junko Hirose …

Science advances, 5(8) eaaw7696, Aug, 2019 Peer-reviewed
Structural basis for broad substrate specificity of UDP-glucose 4-epimerase in the human milk oligosaccharide catabolic pathway of Bifidobacterium longum

Young Woo Nam, Mamoru Nishimoto, Takatoshi Arakawa, Motomitsu Kitaoka, Shinya Fushinobu

Scientific reports, 9(1) 11081, Jul 31, 2019 Peer-reviewed

Misc.

176

Mucin O-glycan assimilation pathway in Bifidobacterium

CHOI Moonhak, 後藤愛那, 阪中幹祥, 加藤紀彦, 西本完, 杉山友太, 栗原新, 吹谷智, 横田篤, 北岡本光 …

日本農芸化学会大会講演要旨集(Web), 2021, 2021
Analysis of mechanism of selective promotion of growth in bifidobacteria with next generation prebiotic Galactosyl-β1,4-rhamnose

平野里佳, 平野里佳, 阪中幹祥, 阪中幹祥, 杉本直久, 江口省吾, 奈良未沙希, 片山高嶺, 北岡本光, 北岡本光 …

日本農芸化学会大会講演要旨集(Web), 2020, 2020
Evolutionary traits found in fucosyllactose transporters support symbiosis between bifidobacteria and breast-fed infants

阪中幹祥, 阪中幹祥, 栗原新, 栗原新, 片山高嶺, 片山高嶺, HANSEN Morten Ejby, 後藤愛那, 加藤紀彦, 吉田圭佑 …

Institute for Fermentation, Osaka. Research Communications, (33), 2019
ビフィズス菌を特異的に増殖させる次世代型プレバイオティクスの開発と応用

平野里佳, 阪中幹祥, 杉本直久, 江口省吾, 奈良未沙希, 片山高嶺, 北岡本光, 中井博之, 栗原新

日本乳酸菌学会誌, 30(2), 2019
Human Milk Oligosaccharides as Bifidus Factor

北岡本光

日本調理科学会誌, 52(5) 352‐357(J‐STAGE), 2019
糖質関連酵素高度活用技術の開発

北岡本光

応用糖質科学, 8(1) 20‐32, Feb 20, 2018
Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes

Wade Abbott, Orly Alber, Ed Bayer, Jean-Guy Berrin, Alisdair Boraston, Harry Brumer, Ryszard Brzezinski, Anthony Clarke, Beatrice Cobucci-Ponzano, Darrell Cockburn …

GLYCOBIOLOGY, 28(1) 3-8, Jan, 2018
オリゴ糖実用的調製に有用なホスホリラーゼ

北岡本光

応用糖質科学, 7(2) 104‐108-108, May 20, 2017
酵素工学を活用した糖質資源高度利用プラットフォームの構築

北岡本光

JATAFジャーナル, 5(4) 45-45, Apr, 2017
メタゲノムを活用した新規ホスホリラーゼスクリーニング法の構築

柴田理沙, 佐藤耕平, 杉本直久, 仁平高則, 北岡本光, 松沢智彦, 末永光, 矢追克郎, 片山高嶺, 中井博之

応用糖質科学, 7(3), 2017
2-O-α-D-グルコシル-D-グリセリン酸ホスホリラーゼ

五十嵐充, 間島滉一郎, 仁平高則, 北岡本光, 荒川孝俊, 伏信進矢, 中井博之, 中井博之

応用糖質科学, 7(3), 2017
β-1,2-グルカン関連酵素群の機能と構造

中島将博, 阿部紘一, 阿部紘一, 田中信清, 宮永顕正, 中井博之, 伏信進矢, 五十嵐圭日子, 北岡本光, 鮫島正浩 …

応用糖質科学, 7(3), 2017
酵素耐熱化の実例―ミルクオリゴ糖合成酵素の耐熱化―

北岡本光

New Food Ind, 58(4) 33-37, Apr 1, 2016
Ruminococcus albus由来4‐O‐β‐D‐マンノシル‐D‐グルコースホスホリラーゼのサブサイト+1に保存されるArg92の基質結合への関与

塩田咲耶子, 尾高伶, 佐分利亘, YE Yuxin, 薦田圭介, 加藤公児, 西本完, 北岡本光, YAO Min, 森春英

日本農芸化学会大会講演要旨集(Web), 2016 4D009 (WEB ONLY), Mar 5, 2016
ホスホリラーゼを用いたスクロースからのセルロース合成

久我友大, 砂川直輝, 石田卓也, 竹内美由紀, 五十嵐圭日子, 鮫島正浩, 北岡本光

セルロース学会年次大会講演要旨集, 23rd, 2016
Ap-8 マウス脾臓細胞のサイトカイン産生に及ぼすニゲロースの影響(その他有用糖質,一般講演,一般社団法人日本応用糖質科学会平成27年度大会(第64回))

杉本直久, 伊藤紗織, 佐藤佳太, 仁平高則, 大坪研一, 北岡本光, 原崇, 中井博之

応用糖質科学 : 日本応用糖質科学会誌, 5(3) B39, Aug 20, 2015
Ba-10 Bifidobacterium longum由来酢酸キナーゼの機能解析(ホスホリラーゼ関連,一般講演,一般社団法人日本応用糖質科学会平成27年度大会(第64回))

劉遠, 西本完, 北岡本光

応用糖質科学 : 日本応用糖質科学会誌, 5(3) B42, Aug 20, 2015
Ruminococcus albus由来4‐O‐β‐D‐mannosyl‐D‐glucose phosphorylase RaMP1の無機リン酸に対するホモトロピック相互作用

尾高伶, 佐分利亘, YE Yuxin, 薦田圭介, 加藤公児, 西本完, 北岡本光, 姚閔, 森春英

応用糖質科学, 5(3) 42, Aug 20, 2015
Ba-9 Ruminococcus albus由来4-O-β-D-mannosyl-D-glucose phosphorylase RaMP1の無機リン酸に対するホモトロピック相互作用(ホスホリラーゼ関連,一般講演,一般社団法人日本応用糖質科学会平成27年度大会(第64回))

尾高伶, 佐分利亘, Ye Yuxin, 薦田圭介, 加藤公児, 西本完, 北岡本光, 姚閔, 森春英

応用糖質科学 : 日本応用糖質科学会誌, 5(3) B42, Aug 20, 2015
新規β‐マンノシドホスホリラーゼの発見

知久和寛, 仁平高則, 鈴木絵里香, 西本完, 北岡本光, 中井博之, 大坪研一

応用糖質科学, 5(2) 120-127, May 20, 2015

Research Projects

Comprehensive analysis and application of a bacterial Levoglucosan metabolism involving novel enzymes

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2021 - Mar, 2025
Investigation for unrevealed lytic pathway of polysaccharides initiated with oxidation of a hydroxyl group

Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Exploratory), Japan Society for the Promotion of Science, Jul, 2021 - Mar, 2024
Enzymatic insight into the microbiota formation in infant guts

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2021 - Mar, 2024

Industrial Property Rights

特開2018-108968 腸内細菌叢改善剤及びその使用

中井博之, 栗原新, 片山高嶺, 北岡本光
特開2018-082636 微生物を用いたポリオールエステルの製造方法

真野潤一, 北岡本光, 池正和, 徳安健, 橘田和美, 小野裕嗣
特開2017-163881 オリゴ糖の製造方法

北岡本光, 西本完
特許第6171598号 β−マンノシドの製造方法

中井博之, 仁平高則, 大坪研一, 北岡本光
特開2017-000002 グリコーゲンホスホリラーゼ製剤の製造方法

北岡本光, 清水金忠
特開2014-239651 β−マンノシドの製造方法

中井博之, 仁平高則, 大坪研一, 北岡本光
特開2014-210718 腸管バリア機能改善剤

北岡本光, 西本完, 佐藤拓海, 清水金忠, 武藤正達, 元吉智美
特開2014-187886 1,3−βガラクトシル−N−アセチルヘキソサミンホスホリラーゼの製造方法

北岡本光
特開2014-168404 耐熱性1,3−βガラクトシル−N−アセチルヘキソサミンホスホリラーゼ

小山善幸, 西本完, 北岡本光
特開2014-128206 グルコシル−α−1,2−グリセロールホスホリラーゼ及びそれを用いたグルコシル−α−1,2−グリセロールの製造方法

中井博之, 仁平高則, 斉藤由華, 大坪研一, 北岡本光
特開2014-079185 セロビオン酸ホスホリラーゼ及びそれを用いた酸性βグルコシル二糖の製造方法

中井博之, 仁平高則, 斉藤由華, 大坪研一, 北岡本光, 西本完
特開2014-057534 オリゴ糖合成酵素並びにβ−1,2−マンノビオース及びその誘導体の製造方法

中井博之, 仁平高則, 鈴木絵里香, 大坪研一, 北岡本光, 西本完
特開2014-045704 オリゴ糖合成酵素およびアスパラギン結合型糖タンパク質のコア糖鎖構造の製造方法

中井博之, 仁平高則, 鈴木絵里香, 大坪研一, 北岡本光
特開2012-254061 ニゲロースホスホリラーゼ

仁平高則, 中井博之, 北岡本光
特開2012-095606 デキストラングルカナーゼ

舟根和美, 北岡本光, 宮城貞夫, 儀部茂八
WO2011-096360 ラクト−N−ビオース含有液の処理方法

北岡本光, 西本完, 清水金忠
特開2011-116725 抗肥満剤、抗肥満用飲食品

北岡本光, 近藤しずき, 清水金忠, 小田巻俊孝, 岩淵紀介
特開2009-027971 Nアセチルガラクトサミンの製造方法

井上公輔, 西本完, 北岡本光
特開2008-290972 ビフィズス菌選択的増殖促進剤

北岡本光, 西本完
特開2008-167744 サイクロデキストランの製造方法およびサイクロデキストラン合成酵素の製造方法

舟根和美, 北岡本光, 川端康之, 宮城貞夫, 儀部茂八

Profile Information

Research Interests

Research Areas

Research History

Awards

Papers

Misc.

Research Projects

Industrial Property Rights

Menu

Coauthors