2013年
Real-time monitoring of changes in microtubule mechanical properties in response to microtubule-destabilizing drug treatment
Journal of Nanoscience and Nanotechnology
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- ,
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- 巻
- 13
- 号
- 3
- 開始ページ
- 2087
- 終了ページ
- 2090
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1166/jnn.2013.6876
- 出版者・発行元
- AMER SCIENTIFIC PUBLISHERS
Microtubules are cylindrical protein polymers that play important roles in a number of cellular functions. The properties of microtubules are dynamically changed by interacting with many microtubule-related proteins and drugs. In this study, we used atomic force microscopy to evaluate the changes in microtubule mechanical properties induced by treatment with nocodazole, which is a microtubule-destabilizing drug. The average spring constant of the microtubules, which was used as a measure of microtubule lateral stiffness, was drastically decreased by treatment with nocodazole within 30 min from 0.052 +/- 0.014 N/m to 0.029 +/- 0.015 N/m. Our findings will aid in the understanding of microtubule dynamics, protein interactions in response to drug treatment, microtubule-related diseases, and drug development
- リンク情報
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- DOI
- https://doi.org/10.1166/jnn.2013.6876
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000319027300086&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-84876258520&partnerID=MN8TOARS
- ID情報
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- DOI : 10.1166/jnn.2013.6876
- ISSN : 1533-4880
- eISSN : 1533-4899
- ORCIDのPut Code : 38324007
- SCOPUS ID : 84876258520
- Web of Science ID : WOS:000319027300086