Yashuo Shirakihara

Profile Information

Affiliation: 助教授, 構造遺伝学研究センター, 国立遺伝学研究所

Degree: Ph D(The University of Tokyo)

J-GLOBAL ID: 200901078938423056
researchmap Member ID: 1000052208

Research Interests

Research Areas

Life sciences / Biophysics /

Papers

Formation of repressor-inducer-operator ternary complex: negative cooperativity of d-camphor binding to CamR

Hironori Aramaki, Hiroyuki Kabata, Shuso Takeda, Hiroshi Itou, Hideki Nakayama, Nobuo Shimamoto

GENES TO CELLS, 16(12) 1200-1207, Dec, 2011 Peer-reviewed
Crystal Structures of the Multidrug Binding Repressor Corynebacterium glutamicum CgmR in Complex with Inducers and with an Operator

Hiroshi Itou, Nobuhisa Watanabe, Min Yao, Yasuo Shirakihara, Isao Tanaka

JOURNAL OF MOLECULAR BIOLOGY, 403(2) 174-184, Oct, 2010 Peer-reviewed
Crystal structure of salt-tolerant glutaminase from Micrococcus luteus K-3 in the presence and absence of its product L-glutamate and its activator Tris

Kazuaki Yoshimune, Yasuo Shirakihara, Mamoru Wakayama, Isao Yumoto

FEBS JOURNAL, 277(3) 738-748, Feb, 2010 Peer-reviewed
Structural and functional characterization of the LldR from Corynebacterium glutamicum: a transcriptional repressor involved in L-lactate and sugar utilization

Yong-Gui Gao, Hiroaki Suzuki, Hiroshi Itou, Yong Zhou, Yoshikazu Tanaka, Masaaki Wachi, Nobuhisa Watanabe, Isao Tanaka, Min Yao

NUCLEIC ACIDS RESEARCH, 36(22) 7110-7123, Dec, 2008 Peer-reviewed
Structural basis for tropomyosin overlap in thin (actin) filaments and the generation of a molecular swivel by troponin-T

Kenji Murakami, Murray Stewart, Kayo Nozawa, Kumiko Tomii, Norio Kudou, Noriyuki Igarashi, Yasuo Shirakihara, Soichi Wakatsuki, Takuo Yasunaga, Takeyuki Wakabayashi

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 105(20) 7200-7205, May, 2008 Peer-reviewed
Crystal structure of the PH1932 protein, a unique archaeal ArsR type winged-HTH transcription factor from Pyrococcus horikoshii OT3

Hiroshi Itou, Min Yao, Nobuhisa Watanabe, Isao Tanaka

Proteins: Structure, Function and Genetics, 70(4) 1631-1634, Mar, 2008 Peer-reviewed
The structures of transcription factor CGL2947 from Corynebacterium glutamicum in two crystal forms: A novel homodimer assembling and the implication for effector-binding mode

Yong-Gui Gao, Min Yao, Hiroshi Itou, Yong Zhou, Isao Tanaka

PROTEIN SCIENCE, 16(9) 1878-1886, Sep, 2007 Peer-reviewed
Crystal structure of a major fragment of the salt-tolerant glutaminase from Micrococcus luteus K-3

Kazuaki Yoshimune, Yasuo Shirakihara, Aya Shiratori, Mamoru Wakayama, Panuwan Chantawannakul, Mitsuaki Moriguchi

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 346(4) 1118-1124, Aug, 2006 Peer-reviewed
Entropically driven MHC class I recognition by human inhibitory receptor leukocyte Ig-like receptor B1 (LILRB1/ILT2/CD85j)

M Shiroishi, K Kuroki, K Tsumoto, A Yokota, T Sasaki, K Amano, T Shimojima, Y Shirakihara, L Rasubala, PA van der Merwe …

JOURNAL OF MOLECULAR BIOLOGY, 355(2) 237-248, Jan, 2006 Peer-reviewed
The CGL2612 protein from Corynebacterium glutamicum is a drug resistance-related transcriptional repressor - Structural and functional analysis of a newly identified transcription factor from genomic DNA analysis

H Itou, U Okada, H Suzuki, M Yao, M Wachi, N Watanabe, Tanaka, I

JOURNAL OF BIOLOGICAL CHEMISTRY, 280(46) 38711-38719, Nov, 2005 Peer-reviewed
Expression, crystallization and preliminary diffraction studies of the Pseudomonas putida cytochrome P450cam operon repressor CamR

K Maenaka, K Fukushi, H Aramaki, Y Shirakihara

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, 61 796-798, Aug, 2005 Peer-reviewed
Efficient large-scale protein production of larvae and pupae of silkworm by Bombyx mori nuclear polyhedrosis virus bacmid system

T Motohashi, T Shimojima, T Fukagawa, K Maenaka, EY Park

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 326(3) 564-569, Jan, 2005 Peer-reviewed
Structure analysis of PH1161 protein, a transcriptional activator TenA homologue from the hyperthermophilic archaeon Pyrococcus horikoshii

H Itou, M Yao, N Watanabe, Tanaka, I

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 60 1094-1100, Jun, 2004 Peer-reviewed
F0F1-ATPase/synthase is geared to the synthesis mode by conformational rearrangement of epsilon subunit in response to proton motive force and ADP/ATP balance

T Suzuki, T Murakami, R Iino, J Suzuki, S Ono, Y Shirakihara, M Yoshida

JOURNAL OF BIOLOGICAL CHEMISTRY, 278(47) 46840-46846, Nov, 2003 Peer-reviewed
Human inhibitory receptors Ig-like transcript 2 (ILT2) and ILT4 compete with CD8 for MHC class I binding and bind preferentially to HLA-G

M Shiroishi, K Tsumoto, K Amano, Y Shirakihara, M Colonna, VM Braud, DSJ Allan, A Makadzange, S Rowland-Jones, B Willcox …

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 100(15) 8856-8861, Jul, 2003 Peer-reviewed
Crystal structure of Enterococcus hirae enolase at 2.8 angstrom resolution

T Hosaka, T Meguro, Yamato, I, Y Shirakihara

JOURNAL OF BIOCHEMISTRY, 133(6) 817-823, Jun, 2003 Peer-reviewed
Crystallization and preliminary X-ray crystallographic studies of salt-tolerant glutaminase from Micrococcus luteus K-3

P Chantawannakul, K Yoshimune, Y Shirakihara, A Shiratori, M Wakayama, M Moriguchi

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 59 566-568, Mar, 2003 Peer-reviewed
The human low affinity Fc gamma receptors IIa, IIb, and III bind IgG with fast kinetics and distinct thermodynamic properties

K Maenaka, PA van der Merwe, DI Stuart, EY Jones, P Sondermann

JOURNAL OF BIOLOGICAL CHEMISTRY, 276(48) 44898-44904, Nov, 2001 Peer-reviewed
Detection of autoantibodies to killer immunoglobulin-like receptors using recombinant fusion proteins for two killer immunoglobulin-like receptors in patients with systemic autoimmune diseases

T Matsui, M Otsuka, K Maenaka, H Furukawa, T Yabe, K Yamamoto, K Nishioka, T Kato

ARTHRITIS AND RHEUMATISM, 44(2) 384-388, Feb, 2001 Peer-reviewed

Misc.

Draft genome sequence of a thermophilic member of the Bacillaceae, Anoxybacillus flavithermus strain Kn10, isolated from the Kan-nawa Hot Spring in Japan

Minenosuke Matsutani, Yasuo Shirakihara, Katsumi Imada, Toshiharu Yakushi, Kazunobu Matsushita

Genome Announcements, 1(3) e00311-e00313, 2013
Crystallization and preliminary X-ray diffraction studies on the DNA-binding domain of the transcriptional activator protein PhoB from Escherichia coli

K Shindoh, K Maenaka, T Akiba, H Okamura, Y Nishimura, K Makino, Y Shirakihara

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 58 1862-1864, Oct, 2002
Crystallization of the F41 fragment of flagellin and data collection from extremely thin crystals

FA Samatey, K Imada, F Vonderviszt, Y Shirakihara, K Namba

JOURNAL OF STRUCTURAL BIOLOGY, 132(2) 106-111, Nov, 2000
ATP合成酵素

蛋白質核酸酵素, 44(4) 538-545, 1999
ATP synthase

44(4) 538-545, 1999
The crystal structure of the nucleotide-free alpha 3 beta 3 subcomplex of F-1-ATPase from the thermophilic Bacillus PS3 is a symmetric trimer

Y Shirakihara, AGW Leslie, JP Abrahams, JE Walker, T Ueda, Y Sekimoto, M Kambara, K Saika, Y Kagawa, M Yoshida

STRUCTURE, 5(6) 825-836, Jun, 1997
Crystallization of 31 KDa C-terminal Fragment of Rat DXIA Dalymerase β

Journal of Molecular Biology, 235(4) 1342-1344, 1994
好熱菌F<D2>1</D2>-ATPaseのα<D2>3</D2>β<D2>3</D2>複合体の結晶化

日本生物物理学会, 33(S) 87, 1993
Crystallization of α<D2>3</D2>β<D2>3</D2> complex of F<D2>1</D2> ATPase from a Thermophilic Bacterium.

Japanese Biophysics Society, 33(S) 87, 1993
好熱菌F1-ATPaseとそのサブユニットの精製と結晶化

109, 1991
Purification by Dye-Ligana Chromatography and a Crystallization Study of F1-ATPase and its Major Subumits, Bandα, from a Thermophilic Bacterium PS3

Journal of Biochemistry, 109, 1991
大腸菌ホスホフルクトキナーゼとその産物の複合体の結晶構造

204, 1988
The Crystal Structure of the Complex of Phosphofructakinase from E. coli with its Reaction Products

Journal of Molecular Biology, 204, 1988

Books and Other Publications

膜タンパク質の結晶解析

東京化学同人, 1992
X-ray Crystal Analysis of Membrane Proteins

Tokyo Kagaku-Dojin, 1992

Presentations

Crystallization of ATPsynthase

Shirakihara,Y, Tanikawa,H, Murakami,S

Oct 29, 2013
複製開始因子ColE2-Rep DNA結合領域と複製開始点との複合体の結晶構造解析：プライマーゼ活性を持つユニークなRepタンパク質による特異的ori認識ならびに2本鎖DNA解裂機構の構造学的研究

伊藤啓, 矢倉勝, 伊藤建夫, 白木原康雄

第35回日本分子生物学会年会, Dec 11, 2012
Structure analysis of the DNA-binding domain of ColE2-Rep in complex with the replication origin

Itou, H, Yagura, M, Itoh, T, Shirakihara, Y

A joint meeting of the Asian Crystallographic Association, Society of Crystallographers in Australia and New Zealand, Dec 2, 2012
複製開始因子ColE2-Repによる複製開始点の認識ならびに2本鎖開裂機構の構造学的研究

伊藤啓, 矢倉勝, 伊藤建夫, 白木原康雄

日本結晶学会2012年年会, Oct 25, 2012
Crystallization of ATPsynthase

Shirakihara,Y, Tanikawa,H, Murakami,S

Sep 22, 2012
複製開始因子ColE2-Repによるori認識機構の構造生物学的研究

伊藤啓, 矢倉勝, 伊藤建夫, 白木原康雄

日本結晶学会2011年年会, Nov 24, 2011
Structure of Salt-tolerant Glutaminase:Structural Basis for Salt Tolerance

Yoshimune,K&Shirakihara,Y

生物物理学会49回年会, Sep 18, 2011
Crystallization of ATPsynthase

Shirakihara, Y, Tanikawa, H, Yoshimune,K, Murakami S, Suzuki T, Yoshida M

Sep 21, 2010
Crystallization of ATPsynthase

Shirakihara, Y, Tanikawa, H, Yoshimune,K, Murakami S, Suzuki T, Yoshida M

Oct 30, 2009
C.glutamicum由来多剤調節因子CgmRの構造学的研究：多剤耐性に適したもうひとつのリプレッサーの作用機構

Itou, H, Watanabe, N, Yao, M, Shirakihara, Y, Tanaka, I

日本蛋白質科学会2009年年会, May 20, 2009
Crystallization and crystal analysis of ATPsynthase

Shirakihara, Y, Shiratori A, Murakami S, Suzuki T, Yoshida M

IUCr2008, Aug 23, 2008
Structural studies of the multidrug-responsive transcriptional repressor protein CgmR

Itou, H, Watanabe, N, Yao, M, Shirakihara, Y, Tanaka, I

21st congress and general assembly of the international union of crystallography, Aug 23, 2008
Crystallization and crystal analysis of ATPsynthase

Shirakihara, Y, Shiratori A, Murakami S, Suzuki T, Yoshida M

Dec 21, 2007
転写開始におけるRNA polymeraseの活性型と不活性型のスイッチ機構

今清水正彦, 伊藤啓, 田中寛, 村上勝彦, 嶋本伸雄

生物物理学会年会2007, Dec 21, 2007
シアノバクテリアRNA polymeraseにおける転写開始のスイッチ機構

今清水正彦, 伊藤啓, 田中寛, 村上勝彦, 嶋本伸雄

BMB2007, Dec 11, 2007
Corynebacterium glutamicum由来蛋白質CGL2915の構造と機能解析：乳酸と炭水化物利用に関わる新規転写リプレッサー

Gao, YG, Yao, M, Suzuki, M, Itou, H, Wachi, M, Watanabe, N, Tanaka, I

日本蛋白質科学会2007年会, May 24, 2007
バクテリアの多剤耐性調節転写因子CGL2612タンパク質による、薬剤・DNA認識機構の構造学的研究

Itou, H, Suzuki, M, Wachi, M, Watanabe, N, Tanaka, I

日本蛋白質科学会2007年年会, May 24, 2007
Improvement and analysis of ATP synthase crystals

Shirakihara, Y, Shiratori A, Murakami S, Suzuki T, Yoshida M

EABS & BSJ 2006, Nov 12, 2006
The CGL2612 protein, a newly identified protein by genomic DNA analysis on Corynebacterium glutamicum, is a drug resistance-related transcriptional repressor

Itou, H, Okada, U, Suzuki, H, Wachi, M, Yao, M, Watanabe, N, Shirakihara, Y, Tanaka, I

20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress, Jun 18, 2006
タンパク3000プロジェクト「転写・翻訳」拠点の成果（５）有用細菌由来転写因子の立体構造解析

Gao, YG, Hayashi, T, Sakai, N, Itou, H, Yao, M, Watanabe, N, Tamura, T, Tanaka, I

日本蛋白質科学会2006年年会, Apr 24, 2006

Professional Memberships

Works

F1-ATPase部分複合体の構造解析

1994 - 1997
Structure Analysis of subcompler of F1-ATPase

1994 - 1997
好熱菌F1-ATPaseのX線構造解析

1986
X-ray Structure Analysis of thermophilic F1-ATPase

1986

Profile Information

Research Interests

Research Areas

Papers

Misc.

Books and Other Publications

Presentations

Professional Memberships

Works

Research Projects

Menu