1997年2月
Efficient removal of loxP-flanked DNA sequences in a gene-targeted locus by transient expression of cre recombinase in fertilized eggs
Molecular Reproduction and Development
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- 巻
- 46
- 号
- 2
- 開始ページ
- 109
- 終了ページ
- 113
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/(SICI)1098-2795(199702)46:2<109::AID-MRD1>3.0.CO;2-U
The bacteriophage P1 Cre/loxP site-specific recombination system is a useful tool for engineering chromosomal changes in animal cells. Transient expression of the Cre recombinase gene directly introduced into fertilized eggs by pronuclear injection has been reported to provide an efficient method of transgene modulation in fertilized eggs. In the present study, we examined the efficacy of this method to remove loxP-flanked DNA sequences in a gene- targeted locus in fertilized eggs. We replaced a part of the T-cell receptor γ (TCR Vγ) locus with homologous sequences containing a loxP-flanked neogene in mouse embryonic stem (ES) cells by gene-targeting technique. The resulting ES cell clones containing the mutant allele (Vγ(LNL)) were used to generate chimeric mice by blastocyst injection. Eight male chimeras were bred with superovulated wild-type female mice. One hundred and seventy-six fertilized eggs were collected, and subjected to pronuclear injection of the Cre expression plasmid, pCAGGS-Cre, of a covalently closed circular form. Three out of 11 pups inherited the targeted Vγ locus. The inherited targeted allele of these 3 mice was shown to have undergone Cre-mediated recombination, resulting in a deletion of the loxP-flanked sequences (Vγ(Δ)) as shown by Southern blot analysis of DNA from tail biopsies. All 3 founder mutant mice were capable of transmitting the Vγ(Δ) locus to their offspring. The other 8 pups carried only wild-type alleles. There were no pups carrying the unrecombined Vγ(LNL) locus. Thus, the frequency of Cre- mediated recombination was 100% (3/3) with this method. In contrast, when closed circular pCAGGS-Cre plasmid was introduced into ES cells by electroporation, the recombination frequency of the Vγ(LNL) locus was 9.6%. These results indicated that our system based on transient expression of the Cre recombinase gene directly introduced into fertilized eggs by pronuclear injection provides a fast and efficient method for generating mutant mice with desired deletions or translocations in target genes.
- リンク情報
- ID情報
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- DOI : 10.1002/(SICI)1098-2795(199702)46:2<109::AID-MRD1>3.0.CO;2-U
- ISSN : 1040-452X
- PubMed ID : 9021742
- SCOPUS ID : 0031019964