1997年12月
Role of cytosolic phospholipase A(2) in allergic response and parturition
NATURE
- 巻
- 390
- 号
- 6660
- 開始ページ
- 618
- 終了ページ
- 622
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/37622
- 出版者・発行元
- MACMILLAN MAGAZINES LTD
Phospholipase A(2) (PLA(2)) comprises a superfamily of enzymes that hydrolyse the ester bond of phospholipids at the sn-2 position(1-3). Among the members of this superfamily, cytosolic PLA(2) has attracted attention because it preferentially hydrolyses arachidonoyl phospholipids and is activated by submicromolar concentrations of Ca2+ ions and by phosphorylation by mitogen-activated protein kinases (MAP kinases)(4-8). Here we investigate the function of cytosolic PLA(2) in vivo by using homologous recombination to generate mice deficient in this enzyme. These mice showed a marked decrease in their production of eicosanoids and platelet-activating factor in peritoneal macrophages. Their ovalbumin-induced anaphylactic responses were significantly reduced, as was their bronchial reactivity to methacholine. Female mutant mice failed to deliver offspring, but these could be rescued by administration of a progesterone-receptor antagonist to the mother at term. Considered together with previous findings(9-15), our results indicate that cytosolic PLA(2) plays a non-redundant role in allergic responses and reproductive physiology.
- リンク情報
- ID情報
-
- DOI : 10.1038/37622
- ISSN : 0028-0836
- Web of Science ID : WOS:A1997YK85300052