2011年2月
Detection of Chlamydophila psittaci by Using SYBR Green Real-Time PCR
JOURNAL OF VETERINARY MEDICAL SCIENCE
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- 巻
- 73
- 号
- 2
- 開始ページ
- 249
- 終了ページ
- 254
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1292/jvms.10-0222
- 出版者・発行元
- JAPAN SOC VET SCI
Chlamydophila psittaci is the causative agent of human psittacosis and avian chlamydiosis. This zoonotic pathogen is frequently transmitted from infected birds to humans. Therefore proper and rapid detection of C. psittaci in birds is important to control this disease. We developed a method for detecting C. psittaci by using SYBR Green Real-time PCR based on targeting the cysteine-rich protein gene (envB) of C. psittaci. This one step procedure was highly sensitive and rapid for detection and quantification of C. psittaci from fecal samples. This assay was also able to detect other zoonotic Chlamydophila species such as C. abortus and C. fells. The assay is well suited for use as a routine detection method in veterinary medicine.
- リンク情報
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- DOI
- https://doi.org/10.1292/jvms.10-0222
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/20948172
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000288289800018&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=79952577447&origin=inward
- ID情報
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- DOI : 10.1292/jvms.10-0222
- ISSN : 0916-7250
- PubMed ID : 20948172
- Web of Science ID : WOS:000288289800018